A Strain of Siniperca chuatsi Rhabdovirus Causes High Mortality among Cultured Largemouth Bass in South China
A Strain of Siniperca chuatsi Rhabdovirus Causes High Mortality among Cultured Largemouth Bass in South China
复制标题
鳜鱼弹状病毒株导致华南养殖大口黑鲈的高死亡率。
DOI：
10.1080/08997659.2013.799613
复制
发表时间：
2013-09-01
影响因子：
1.2
通讯作者：
Ye, Xing
中科院分区：
文献类型：
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作者：
Ma, Dongmei;Deng, Guocheng;Ye, Xing
2011年4月，广东省中山市某养殖场发生大口黑鲈（Micropterus salmoides）幼鱼死亡事件，死亡率高达40%。受感染的鱼变得昏昏欲睡，表现出螺旋形和不规则的游泳，并开发了一个膨胀的腹部和弯曲的身体。在出现临床症状后2 d内，鱼开始死亡。为分析病原，早期诊断该病，进行了临床症状观察、细胞感染、病毒滴度计算、电镜观察、浸泡感染试验和核苷酸序列分析。用病鱼肝、脾匀浆滤液接种黑头呆鱼细胞培养物，在原代培养46 h和第1代24 h时，出现明显的细胞病变。电镜下观察到典型的弹状病毒颗粒，长115- 143 nm，直径62- 78 nm。分离的病毒产生的滴度为10（7.15）TCID 50/mL。感染病毒的浸鱼具有相似的临床症状和80%的死亡率，10（2.5）LD 50/mL。这些资料表明，弹状病毒是目前该病的致死性病原。根据核蛋白-基因核苷酸序列多重比对分析，新分离的病毒为一株来源于中华鲟的弹状病毒，属于弹状病毒科（Rhabdoviridae）。迄今为止，已从患病的大口黑鲈中分离到至少4株病毒，它们具有不同的临床症状。临床症状的比较有助于疾病的早期诊断。接收日期：2012年10月30日;接受日期：2013年4月19日
In April 2011, 40% mortality of Largemouth Bass Micropterus salmoides juveniles occurred at a farm of Zhongshan City, Guangdong Province, China. Infected fish became lethargic, exhibited corkscrew and irregular swimming, and developed a distended abdomen and crooked body. Fish began to die within 2 d after the appearance of clinical signs. In order to analyze the pathogeny and diagnose the disease earlier, observation of clinical signs, cell infection, titer calculation, electron microscopy, immersion infection assay for fish, and nucleotide sequence analysis were carried out. Fathead minnow (FHM) cell cultures, inoculated with filtrate of liver and spleen homogenates from the diseased fish, developed the obvious cytopathic effect 46h after inoculation in the primary culture and 24h at the first passage. Typical rhabdovirus particles, 115-143nm in length and 62-78nm in diameter, were observed in infected FHM cells by direct transmission electron microscopy. The isolated virus produced a titer of 10(7.15) TCID50/mL. Immersion-Fish infected with the virus had similar clinical signs and 80% mortality with 10(2.5) LD50/mL. The data indicated that the rhabdovirus was the lethal pathogeny of the current disease. Based on nucleoprotein-gene nucleotide sequence multiple alignment analysis, the newly isolated virus is a strain of Siniperca chuatsi rhabdovirus (SCRV) under family Rhabdoviridae, which was initially isolated from Mandarin Fish Siniperca chuatsi. Up to the present, at least four virus strains have been isolated from diseased Largemouth Bass, which have had different clinical signs. Comparison of the clinical signs can help in an early diagnosis of the disease. Received October 30, 2012; accepted April 19, 2013