Genetic Differences in the Osteogenic Differentiation Potency According to the Classification of Ossification of the Posterior Longitudinal Ligament of the Cervical Spine
Genetic Differences in the Osteogenic Differentiation Potency According to the Classification of Ossification of the Posterior Longitudinal Ligament of the Cervical Spine
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DOI：
10.1097/brs.0b013e3181e9a8a6
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发表时间：
2011-05-20
期刊：
影响因子：
3
通讯作者：
Toh, Satoshi
中科院分区：
文献类型：
--
作者：
Kudo, Hitoshi;Furukawa, Ken-Ichi;Toh, Satoshi
研究设计.我们将颈椎后纵韧带骨化的四种类型分为两组。我们用生化方法研究了两组间成骨分化潜能的遗传差异。根据OPLL分类，研究成骨分化潜能的遗传差异。OPLL的临床研究表明，连续型和混合型OPLL的骨化区域进展风险最大。然而，到目前为止，这四种类型的OPLL已被研究作为一个单一的条件。我们将OPLL的四种类型分为OPLL连续型（连续型或混合型）和OPLL节段型（节段型或限制型）。在手术过程中从OPLL患者中无菌获得椎旁韧带。从外植体迁移的成纤维细胞样细胞用于实验。将细胞置于60 mm培养皿中用于总核糖核酸制备，并置于12孔微孔板中用于碱性磷酸酶（ALP）活性染色。在培养物达到汇合后，将细胞在成骨培养基中培养。通过定量真实的时间-聚合酶链反应分析骨形态发生蛋白-2（BMP-2）、osterix、肿瘤坏死因子-α-刺激基因-6和ALP的信使核糖核酸表达。成纤维细胞样细胞成骨分化通过组织化学检测ALP的产生来确定。成骨诱导后，骨形态发生蛋白2的表达增加的OPLL连续和节段性组。Osterix表达仅在OPLL连续组中增加。肿瘤坏死因子-α-刺激基因-6的表达在OPLL连续组和节段组中受到抑制。OPLL连续组ALP表达及活性染色均高于OPLL节段组。研究揭示了OPLL连续组和节段组之间成骨分化潜能的遗传差异。我们建议在OPLL的生化研究中区分OPLL的连续组和节段组。
Study Design. We categorized the four types of ossification of the posterior longitudinal ligament (OPLL) of the cervical spine into two groups. We biochemically investigated the genetic differences in the osteogenic differentiation potency between the two groups.Objective. To investigate the genetic differences in the osteogenic differentiation potency according to the OPLL classification.Summary of Background Data. Clinical studies on OPLL have revealed that the risk of progression of the ossification area is greatest for continuous and mixed type OPLL. However, until now, these four types of OPLL have been studied as a single condition.Methods. We categorized the four types of OPLL into the OPLL continuous (continuous or mixed type) and OPLL segmental groups (segmental or circumscribed type). Paraspinal ligaments were aseptically obtained from OPLL patients during surgery. The fibroblast-like cells that migrated from the explants were used for experiments. The cells were placed in a 60-mm culture dishes for total ribonucleic acid preparation and 12 well microplates for alkaline phosphatase (ALP) activity staining. After cultures reached confluence, the cells were cultured in osteogenic medium. The messenger ribonucleic acid expression of bone morphogenetic protein-2 (BMP-2), osterix, tumor necrosis factor-alpha-stimulated gene-6, and ALP was analyzed by quantitative real time-polymerase chain reaction. Osteogenic differentiation of fibroblast-like cells was determined by histochemically detecting ALP production.Results. After osteogenic induction, BMP-2 expression increased in the OPLL continuous and segmental groups. Osterix expression increased in the OPLL continuous group only. Tumor necrosis factor-alpha-stimulated gene-6 expression was suppressed in the OPLL continuous and segmental groups. ALP expression as well as ALP activity staining was higher in the OPLL continuous group than in the OPLL segmental group.Conclusion. The study revealed genetic differences in the osteogenic differentiation potency between the OPLL continuous and segmental groups. We propose to distinguish OPLL continuous group from segmental group in biochemical studies on OPLL.