Expression of HLA-A, -B, and -C and beta 2-microglobulin antigens in human choriocarcinoma cell lines.
Expression of HLA-A, -B, and -C and beta 2-microglobulin antigens in human choriocarcinoma cell lines.
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HLA-A、-B、-C 和 β2-微球蛋白抗原在人绒毛膜癌细胞系中的表达。
DOI：
10.7326/0003-4819-91-1-147_3
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发表时间：
1980
期刊：
影响因子：
--
通讯作者：
R. Patillo
中科院分区：
文献类型：
--
作者：
J. Trowsdale;P. Travers;W. Bodmer;R. Patillo
[35 S]蛋氨酸标记的细胞提取物的免疫沉淀和分析十二烷基硫酸钠-聚丙烯酰胺凝胶电泳显示，细胞从两个人绒毛膜癌JaR和BeWo含有β 2-微球蛋白。JaR细胞和BeWo的一个亚系不表达表面HLA-A、-B和-C（HLA-ABC）抗原，当在灵敏的放射免疫测定法中测量无细胞提取物时，JaR也不含抗原。可以得出结论，绒毛膜癌（和滋养层）中HLA-ABC抗原表达的缺乏不是缺乏β 2-微球蛋白的结果，如伯基特淋巴瘤细胞系Daudi的情况。
Immunoprecipitation of [35S]methionine-labeled cell extracts and analysis by sodium dodecyl sulfate-polyacrylamide gel electrophoresis showed that cells from two human choriocarcinomas JaR and BeWo contained beta 2-microglobulin. The JaR cells, and one subline of BeWo, did not express surface HLA-A, -B, and -C (HLA-ABC) antigens, nor did JaR contain the antigens when cell-free extracts were measured in a sensitive radioimmunoassay. It may be concluded that the lack of expression of HLA-ABC antigens in choriocarcinoma (and in the trophoblast) is not a result of lack of beta 2-microglobulin, as is the case for the Burkitt's lymphoma cell line, Daudi.