EFFECT OF INSULIN ON UTILIZATION AND PRODUCTION OF CIRCULATING GLUCOSE
EFFECT OF INSULIN ON UTILIZATION AND PRODUCTION OF CIRCULATING GLUCOSE
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DOI：
10.1152/ajplegacy.1957.189.1.43
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发表时间：
1957-01-01
影响因子：
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通讯作者：
KIANG, SP
中科院分区：
文献类型：
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作者：
WALL, JS;STEELE, R;KIANG, SP
给未麻醉的正常犬静脉注射C14葡萄糖后，以使循环葡萄糖的比活度维持在几乎恒定的速率持续给药。不含胰高血糖素的胰岛素随后以不同剂量静脉注射。在整个实验过程中，每隔一段时间采集血液样本，更频繁的是在注射胰岛素之后。循环血液中的葡萄糖浓度和这种葡萄糖的比活度一起考虑时，可以计算a)葡萄糖流出到组织细胞，b)在注射胰岛素后一段时间内从肝脏流入的葡萄糖。通过这一方法表明，在胰岛素诱导的低血糖中，组织对葡萄糖摄取的增加比肝脏葡萄糖输送的减少要重要得多。研究还表明，低血糖的恢复是由肝脏葡萄糖输送的增加带来的，而不是通过组织对低于胰岛素前水平的葡萄糖摄取的减少而实现的。讨论和评价了在血糖浓度变化期间计算葡萄糖流入和流出绝对值的替代方法。
C14glucose was adminisered continuously to unanesthetized normal dogs by intravenous infusion following a priming dose at a rate which maintained the specific activity of the circulating glucose at a nearly constant level. Glucagon-free insulin was then administered intravenously in varying doses. Samples of blood were collected at intervals throughout the experiment and more frequently just subsequent to insulin injection. The glucose concentration of the circulating blood and the specific activity of this glucose, when considered together, allow calculations to be made ofa) the outflow of glucose toward the tissue cells, andb) the inflow of glucose from the liver during the period after insulin injection. By this means it was shown that increased glucose uptake by the tissues is much more important than decreased delivery of glucose by the liver in bringing about insulin-induced hypoglycemia. It was also shown that recovery from hypoglycemia is brought about by increased glucose delivery by the liver and not by a decrease in tissue uptake of glucose below the preinsulin level. Alternative methods of calculation of absolute values for glucose inflow and outflow during periods of changing blood glucose concentrations are discussed and evaluated.