Expression of perilipins in human skeletal muscle in vitro and in vivo in relation to diet, exercise and energy balance
Expression of perilipins in human skeletal muscle in vitro and in vivo in relation to diet, exercise and energy balance
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DOI：
10.3109/13813455.2011.630009
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发表时间：
2012-02-01
影响因子：
3
通讯作者：
Drevon, C. A.
中科院分区：
文献类型：
--
作者：
Gjelstad, I. M. F.;Haugen, F.;Drevon, C. A.
磷脂蛋白包裹细胞内脂滴。我们描述了人体骨骼肌中五种外周蛋白的mRNA表达与脂肪酸供应、运动和能量平衡的关系。我们观察到所有的periilipin在骨骼肌活检中都有表达，其中perilipin 2、4和5的mRNA水平最高。培养的肌管主要表达periilipin 2和perilipin 3。在体外，脂肪酸对肌管的孵育增强了外周血蛋白1、2和4的mRNA表达。在体内，低脂饮食增加了perilipin 3和4的mRNA水平。耐力训练增强了perilipin 2和3的表达，而力量训练不增强。Perilipin 1 mRNA与体脂量呈正相关，而Perilipin均与胰岛素敏感性无关。综上所述，所有periilipins mrna均在人类骨骼肌中表达。饮食和耐力运动可调节perilipin的表达。
The perilipin proteins enclose intracellular lipid droplets. We describe the mRNA expression of the five perilipins in human skeletal muscle in relation to fatty acid supply, exercise and energy balance. We observed that all perilipins were expressed in skeletal muscle biopsies with the highest mRNA levels of perilipin 2, 4 and 5. Cultured myotubes predominantly expressed perilipin 2 and 3. In vitro, incubation of myotubes with fatty acids enhanced mRNA expression of perilipin 1, 2 and 4. In vivo, low fat diet increased mRNA levels of perilipin 3 and 4. Endurance training, but not strength training, enhanced the expression of perilipin 2 and 3. Perilipin 1 mRNA correlated positively with body fat mass, whereas none of the perilipins were associated with insulin sensitivity. In conclusion, all perilipins mRNAs were expressed in human skeletal muscle. Diet as well as endurance exercise modulated the expression of perilipins.