13C metabolic flux analysis in cell line and bioprocess development
13C metabolic flux analysis in cell line and bioprocess development
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DOI：
10.1016/j.coche.2021.100718
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发表时间：
2021-08-17
影响因子：
6.6
通讯作者：
Young, Jamey D.
中科院分区：
文献类型：
--
作者：
Sacco, Sarah A.;Young, Jamey D.
随着对生物制药的需求不断增加，对高产工业细胞系的需求也越来越大。C-13代谢通量分析通过严格量化细胞内代谢网络内的代谢通量，提供了宿主代谢的全面视角。通过代谢工程或培养基优化改变的生产细胞系的特征可以帮助识别所需的代谢表型。再加上高通量微型生物反应器系统的出现和扩展的代谢建模能力，C-13通量分析可以提供细胞内代谢的更完整的图景。由此产生的理想细胞特性和代谢调节节点的知识为系统化识别工程目标提供了新的机会，以开发优化的细胞系和生物过程。
With an ever-increasing demand for biopharmaceuticals, there is a growing need for high-producing industrial cell lines. C-13 metabolic flux analysis provides a comprehensive perspective of host metabolism by rigorously quantifying fluxes within intracellular metabolic networks. The characterization of production cell lines altered via metabolic engineering or media optimization can help to identify desirable metabolic phenotypes. Coupled with the advent of high-throughput mini bioreactor systems and expanded metabolic modeling capabilities, C-13 flux analysis can provide an even more complete picture of intracellular metabolism. The resulting knowledge of desirable cellular traits and metabolic regulatory nodes offers new opportunities for the systematic identification of engineering targets to develop optimized cell lines and bioprocesses.