DIRECT STIMULATION OF CYTOKINES (IL-1-BETA, TNF-ALPHA, IL-6, IL-2, IFN-GAMMA AND GM-CSF) IN WHOLE-BLOOD .1. COMPARISON WITH ISOLATED PBMC STIMULATION
DIRECT STIMULATION OF CYTOKINES (IL-1-BETA, TNF-ALPHA, IL-6, IL-2, IFN-GAMMA AND GM-CSF) IN WHOLE-BLOOD .1. COMPARISON WITH ISOLATED PBMC STIMULATION
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DOI：
10.1016/1043-4666(92)90062-v
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发表时间：
1992-05-01
期刊：
影响因子：
3.8
通讯作者：
FRANCHIMONT, P
中科院分区：
文献类型：
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作者：
DEGROOTE, D;ZANGERLE, PF;FRANCHIMONT, P
用1 × 10稀释的全血（WB）培养液和外周血单个核细胞（PBMC）培养液，研究了脂多糖（LPS）和植物血凝素（PHA）刺激后白细胞介素1 β（IL-1 β）、白细胞介素6（IL-6）、肿瘤坏死因子α（TNF-α）、白细胞介素2（IL-2）、干扰素γ（IFN-γ）和粒细胞-巨噬细胞集落刺激因子（GM-CSF）的产生。细胞因子IL-1 β、TNF-α和IL-6被LPS优先刺激，而IL-2、IFN-γ和GM-CSF被PHA优先刺激。5 μ g/ml PHA和25 μ g/ml LPS的组合产生了所研究的六种细胞因子的最可靠的产生。IL-1 β、TNF-α和IL-6代表了一组同质的早期产生的细胞因子，它们之间以及与培养物中单核细胞的数量（LeuM3）正相关。IL-1 β与T8淋巴细胞数呈负相关。IL-2、IFN-γ和GM-CSF代表一组晚期产生的细胞因子。WB和PBMC培养物中IL-6和GM-CSF的动力学和产生水平相似。相比之下，WB中TNF-α和IFN-γ的产生水平高于PBMC，而WB中IL-6和IL-2的产生水平低于PBMC。在响应PHA + LPS的个体差异总是高于在WB文化的PBMC文化。与单核细胞数量相关的细胞因子产生能力在WB中或在具有与WB相同的单核细胞浓度的PBMC中高于在浓缩PBMC的常规培养物中（10 6/ml）。因为它模拟自然环境，稀释的WB培养物可能是研究体外细胞因子产生的最合适的环境。
Production of interleukin 1β (IL-1β), interleukin 6 (IL-6), tumor necrosis factor α (TNF-α), interleukin 2 (IL-2), interferon gamma (IFN-γ) and granulocyte-macrophage colony-stimulating factor (GM-CSF) after stimulation by lipopolysaccharide (LPS) and phytohemagglutinin (PHA) was studied in 1 10 diluted whole blood (WB) culture and in peripheral blood mononuclear cell (PBMC) culture. Cytokines IL-1β, TNF-α and IL-6 are preferentially stimulated by LPS whereas IL-2, IFN-γ and GM-CSF are stimulated by PHA. Combination of 5 μg/ml PHA and 25 μg/ml LPS gave the most reliable production of the six cytokines studied. IL-1β, TNF-α and IL-6 represent a homogeneous group of early-produced cytokines positively correlated among themselves and with the number of monocytes in the culture (LeuM3). Furthermore, IL-1β was negatively correlated with the number of T8 lymphocytes. IL-2, IFN-γ and GM-CSF represent a group of late-produced cytokines. Kinetics and production levels of IL-6 and GM-CSF are similar in WB and PBMC cultures. In contrast, production levels of TNF-α and IFN-γ are higher in WB than in PBMC whereas production levels of IL-6 and IL-2 are lower in WB than in PBMC. Individual variation in response to PHA+ LPS was always higher in PBMC cultures than in WB cultures. The capacity of cytokine production in relation to the number of mononuclear cells is higher in WB, or in PBMC having the same mononuclear cell concentration as WB, than in conventional cultures of concentrated PBMC (10 6/ml). Because it mimics the natural environment, diluted WB culture may be the most appropriate milieu in which to study cytokine production in vitro.