HIGHLY CONSERVED CORE DOMAIN AND UNIQUE N-TERMINUS WITH PRESUMPTIVE REGULATORY MOTIFS IN A HUMAN TATA FACTOR (TFIID)
HIGHLY CONSERVED CORE DOMAIN AND UNIQUE N-TERMINUS WITH PRESUMPTIVE REGULATORY MOTIFS IN A HUMAN TATA FACTOR (TFIID)
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DOI：
10.1038/346387a0
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发表时间：
1990-07-26
期刊：
影响因子：
64.8
通讯作者：
ROEDER, RG
中科院分区：
文献类型：
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作者：
HOFFMANN, A;SINN, E;ROEDER, RG
TFIID 因子是哺乳动物 RNA 聚合酶 II1,2 转录起始所必需且充分的几个通用因子之一。与常见 TATA 元件 3,4 的稳定相互作用既导致模板承诺，又导致其他一般因子组装成功能性预起始复合物 5,6。与其在启动子激活途径中的关键作用一致，人类 TFIID 似乎也是一些调节因子的目标，这些成分之间相互作用的物理 3,7,8 和功能 19-12 研究证明了这一点。功能特性的进化保守性13,14导致了酵母TFIID15-19的纯化和克隆、推定结构基序的鉴定15,19以及直接的结构-功能研究20。在这里，我们报告了编码功能性人类 TFIID 的互补 DNA 的克隆。这揭示了一个进化保守的核心，它精确对应于酵母 TFIID 确定的 180 个残基 DNA 结合/激活结构域，成分结构基序的近乎绝对保守（直接重复、中央基本核心/赖氨酸重复和 Sigma 同源性），为它们的功能重要性提供了进一步的支持，以及一个独特的 N 末端结构，表明参与物种特异性调节因子相互作用。
THE factor TFIID is one of several general factors that are necessary and sufficient for transcription initiation by mammalian RNA polymerase II1,2. Stable interactions with the common TATA element3,4lead both to template commitment and to the assembly of the other general factors into a functional preinitiation complex5,6. Consistent with its key role in the promoter activation pathway, human TFIID also seems to be a target for some regulatory factors, as evidenced both by physical3,7,8and functionall9–12studies of interactions between these components. The evolutionary conservation of functional properties13,14led to the purification and cloning of yeast TFIID15–19, the identification of presumptive structural motifs15,19, and direct structure–function studies20. Here we report the cloning of a complementary DNA encoding a functional human TFIID. This reveals an evolutionary conserved core which corresponds precisely to the 180-residue DNA binding/activation domain determined20for yeast TFIID, a near absolute conservation of component structural motifs (direct repeats, central basic core/lysine repeat, and sigma homology), providing further support for their functional importance, and a unique N-terminal structure that suggests involvement in species-specific regulatory factor interactions.