The effect of curdlan sulphate on development in vitro of Plasmodium falciparum
The effect of curdlan sulphate on development in vitro of Plasmodium falciparum
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DOI：
10.1016/s0035-9203(98)90969-5
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发表时间：
1998-01-01
影响因子：
2.2
通讯作者：
Havlik, I
中科院分区：
文献类型：
--
作者：
Evans, SG;Morrison, D;Havlik, I
硫酸化糖缀合物已被报道抑制疟疾裂殖子侵入并干扰玫瑰花结和粘附。硫酸可得然胶是一种具有良好毒性的硫酸化糖缀合物，在体外表现出抗疟活性。本研究的目的是表征可得兰的抗疟活性，并研究其对粘附的影响。使用形态学观察和放射性标记的次黄嘌呤摄取作为寄生虫生长的指标，研究了可得兰在红细胞内发育周期中不同点的抗疟活性。使用血小板模型研究对粘附的影响。硫酸可得然胶对寄生虫通过红细胞内循环发育的能力没有影响。对侵袭的抑制依赖于侵袭时存在的药物。在血小板模型中，Curdlan不干扰寄生虫粘附于C36受体的能力。总之，可得然胶的低毒性和其对裂殖子的显著抗侵入活性使得可得然胶成为重症疟疾的潜在辅助治疗。
Sulphated glycoconjugates have been reported to inhibit malarial merozoite invasion and interfere with rosetting and adhesion. Curdlan sulphate, a sulphated glycoconjugate with a favourable toxicity profile, exhibits antimalarial activity in vitro. The aim of this study was to characterize the antimalarial activity of curdlan and investigate its effect on adhesion. The antimalarial activity of curdlan at different points in the intraerythrocytic developmental cycle was investigated using morphological observation and radiolabelled hypoxanthine uptake as indices of parasite growth. Effects on adhesion were investigated using a platelet model. Curdlan suphate had no effect on the ability of the parasite to develop through the intraerythrocytic cycle. Inhibition of invasion was dependent on the drug being present at the time of invasion. Curdlan did not interfere with the ability of the parasite to adhere to the C36 receptor in the platelet model. In conclusion, the low toxicity of curdlan and its marked anti-invasion activity on merozoites make curdlan a potential auxiliary treatment for severe malaria.