Probenazole induces systemic acquired resistance in tobacco through salicylic acid accumulation
Probenazole induces systemic acquired resistance in tobacco through salicylic acid accumulation
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DOI：
10.1006/pmpp.2002.0426
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发表时间：
2002-10-01
影响因子：
2.7
通讯作者：
Yamaguchi, I
中科院分区：
文献类型：
--
作者：
Nakashita, H;Yoshioka, K;Yamaguchi, I
系统获得性抗性（SAR）是植物中一种有效的天然免疫系统，可有效抵抗多种病原体。水杨酸（SA）是介导双子叶植物如烟草和拟南芥SAR发育的重要因子。在拟南芥中SA积累的上游信号通路部分澄清，但在其他植物物种中知之甚少。本研究利用烯丙苯噻唑（probenazole，PBZ）及其活性代谢产物1，2-苯并异噻唑-1，1-二氧化物（berizisothiazole-1，1-dioxide，BIT）研究了烟草SAR信号通路。用PBZ或BIT处理的野生型烟草表现出几种致病相关（PR）基因的表达增加，游离SA和总SA水平增加，以及对病毒病原体烟草花叶病毒（TMV）、细菌病原体烟草假单胞菌致病变种（Pseudomonaserichingae pv.）利用NahG转基因植株分析了SA在PBZ或BIT处理后激活抗性中的作用。PBZ或BIT处理在NahG转基因植物中没有诱导抗病性和PR-1表达，这表明这些化合物通过在SA积累上游的点触发信号传导来诱导SAR。这些结果表明，在双子叶植物物种中，类似的信号通路上游的SA功能。(C)2002爱思唯尔科技有限公司版权所有。
Systemic acquired resistance (SAR) is a potent innate immunity system in plants that is effective against a broad range of pathogens. SAR development in dicotyledonous plant such as tobacco and Arabidopsis is mediated by salicylic acid (SA). The signaling pathway upstream of SA accumulation is partially clarified in Arabidopsis but little is known in other plant species. We investigated the SAR signaling pathway in tobacco using probenazole (PBZ) and its active metabolite, 1,2-berizisothiazole-1,1-dioxide (BIT), which induce SAR in Arabidopsis through SA accumulation. Wild-type tobacco treated with PBZ or BIT exhibited increased expression of several pathogenesis related (PR) genes, increased levels of free and total SA, and enhanced resistance to the viral pathogen tobacco mosaic virus (TMV), the bacterial pathogen Pseudomonas syringae pv. tabaci (Pst), and the fungal pathogen Oidium sp. The role of SA in activating resistance following PBZ or BIT treatment was analysed using NahG transgenic plants. PBZ or BIT treatment did not induce disease resistance and PR-1 expression in NahG transgenic plants, which indicated that these compounds induce SAR by triggering signaling at a point upstream of SA accumulation. These results suggested that similar signaling pathways upstream of SA function in dicotyledonous plants species. (C) 2002 Elsevier Science Ltd. All rights reserved.