Global pattern of interkinetic nuclear migration in tracheoesophageal epithelia of the mouse embryo: Interorgan and intraorgan regional differences
Global pattern of interkinetic nuclear migration in tracheoesophageal epithelia of the mouse embryo: Interorgan and intraorgan regional differences
复制标题
小鼠胚胎气管食管上皮细胞间运动核迁移的整体模式：器官间和器官内区域差异
DOI：
10.1111/cga.12405
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发表时间：
2021
影响因子：
1.3
通讯作者：
Otani Hiroki
中科院分区：
文献类型：
--
作者：
Getachew Dereje;Matsumoto Akihiro;Uchimura Yasuhiro;Udagawa Jun;Mita Nanako;Ogawa Noriko;Moriyama Shigeru;Takami Akiyasu;Otani Hiroki
核间迁移（INM）是上皮干/祖细胞增殖/分化的一种基于顶基底（AB）极性的调控机制。我们先前记录了胚胎第11.5天（E）内胚层来源的气管/食管上皮中的INM，并表明INM参与了两个器官的发育。在这里，我们研究了器官间（气管与食管）和器官内区域（腹侧与背侧）的差异，在INM模式的气管和食管上皮的小鼠胚胎。我们还分析了会聚延伸（CE）和平面细胞运动（PCM）在上皮细胞分布的基础上。在E11.5和E12.5时，向妊娠C57 BL/6 J小鼠腹腔注射5-乙炔基-2 ′-脱氧尿苷，并在1、4、6、8和12 h后处死，以获得胚胎。标记的细胞核的分布沿着AB轴按时间顺序分析的总，腹侧，背侧的上皮。细胞核群的百分比分布用直方图表示，时间变化用多维标度进行统计学分析。在E11.5-E12.0期间INM模式的器官间比较显示出显著差异，但在E12.5-E13.0期间则无此差异。在E11.5-E12.0期间，气管（而非食管）腹侧和背侧之间存在显著差异。在E12.5-E13.0期间，两个器官均未显示区域差异。在E11.5-E12.0期间，两个器官中似乎都发生了CE，而两个器官中的PCM均不清楚。这些发现表明气管和食道之间存在差异，并且在INM模式下，气管而不是食道存在区域差异，这可能与这些器官后来的差异器官发生/组织发生有关。
Interkinetic nuclear migration (INM) is an apicobasal (AB) polarity‐based regulatory mechanism of proliferation/differentiation in epithelial stem/progenitor cells. We previously documented INM in the endoderm‐derived tracheal/esophageal epithelia at embryonic day (E) 11.5 and suggested that INM is involved in the development of both organs. We here investigated interorgan (trachea vs esophagus) and intraorgan regional (ventral vs dorsal) differences in the INM mode in the tracheal and esophageal epithelia of the mouse embryo. We also analyzed convergent extension (CE) and planar cell movement (PCM) in the epithelia based on cell distribution. The pregnant C57BL/6J mice were intraperitoneally injected with 5‐ethynyl‐2′‐deoxyuridine at E11.5 and E12.5 and were sacrificed 1, 4, 6, 8, and 12 hours later to obtain the embryos. The distribution of labeled cell nuclei along the AB axis was chronologically analyzed in the total, ventral, and dorsal sides of the epithelia. The percentage distribution of the nuclei population was represented by histogram and the chronological change was analyzed statistically using multidimensional scaling. The interorgan comparison of the INM mode during E11.5‐E12.0, but not E12.5‐E13.0, showed a significant difference. During E11.5‐E12.0 the trachea, but not the esophagus, showed a significant difference between ventral and dorsal sides. During E12.5‐E13.0 neither organ showed regional differences. CE appeared to occur in both organs during E11.5‐E12.0 while PCM was unclear in both organs. These findings suggest a difference between the trachea and esophagus, and a regional difference in the trachea, not in the esophagus, in the INM mode, which may be related with the later differential organogenesis/histogenesis of these organs.