GENOMIC POLYMORPHISM, GROWTH-PROPERTIES, AND IMMUNOLOGICAL VARIATIONS IN HUMAN HERPESVIRUS-6 ISOLATES
GENOMIC POLYMORPHISM, GROWTH-PROPERTIES, AND IMMUNOLOGICAL VARIATIONS IN HUMAN HERPESVIRUS-6 ISOLATES
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DOI：
10.1016/0042-6822(91)90424-a
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发表时间：
1991-10-01
期刊：
影响因子：
3.7
通讯作者：
GALLO, RC
中科院分区：
文献类型：
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作者：
ABLASHI, DV;BALACHANDRAN, N;GALLO, RC
研究了15株人疱疹病毒6型（HHV-6）分离物对新鲜人脐带血单个核细胞的嗜性，在连续T细胞系中的生长，对单克隆抗体的反应性，和限制酶带型。所有分离株在人脐带血单核细胞中有效复制，但需要在感染前对细胞进行有丝分裂原刺激。感染连续T细胞系的能力因分离株而异。与GS原型相似的分离物感染HSB 2和Sup T1细胞而不感染Molt-3细胞，而与Z-29相似的分离物感染Molt-3细胞而不感染HSB 2和Sup T1细胞。部分抗HHV-6（GS）的单克隆抗体与所有供试分离株均呈反应性，而另一些单克隆抗体仅与类似于GS分离株的HHV-6分离株反应，而不与类似于Z-29分离株的HHV-6分离株反应。用EcoR Ⅰ、BamH Ⅰ和HindIII酶切分析表明，从玫瑰疹、骨髓移植、白细胞减少症和扎伊尔HIV-1阳性艾滋病患者（Z-29）分离的HHV-6与GS型HHV-6分离株密切相关，但不同。根据上述结果，我们认为，与单纯疱疹病毒1型和2型一样，所分析的15株HHV-6分离株可分为A组（GS型）和B组（Z-29型）。
Fifteen human herpesvirus-6 (HHV-6) isolates from normal donors and patients with AIDS, systemic lupus erythmatosis, chronic fatigue syndrome, collagen-vascular disease, leukopenia, bone marrow transplants,Exanthem subitum(roseola), and atypical polyclonal lymphoprolfferation were studied for their tropism to fresh human cord blood mononuclear cells, growth in continuous T cell lines, reactivity to monoclonal antibodies, and by restriction enzyme banding patterns. All isolates replicated efficiently in human cord blood mononuclear cells, but mitogen stimulation of the cells prior to infection was required. The ability to infect continuous T-cell lines varied with the isolates. Isolates similar to GS prototype infected HSB2 and Sup T1 cells and did not infect Molt-3 cells, whereas isolates similar to Z-29 infected Molt-3 cells but not HSB2 and Sup Ti cells. Some of the monoclonal antibodies directed against the HHV-6 (GS) isolate showed reactivity with all isolates tested, but others only reacted with HHV-6 isolates similar to the GS isolate and not with those similar to Z-29 isolate. Restriction enzyme analysis usingEcoRI,BamHI, andHindIII revealed that HHV-6 isolates from roseola, bone marrow transplant, leukopenia, and an HIV-1-positive AIDS patient from Zaire (Z-29) were closely related but distinct from GS type HHV-6 isolates. Based on the above findings, we propose that, like herpes simplex virus types 1 and 2, the 15 HHV-6 isolates analyzed can be divided into group A (GS type) and group B (Z-29 type).