Cellular genesis in the postnatal piglet
Cellular genesis in the postnatal piglet
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DOI：
10.1016/j.ijdevneu.2008.04.001
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发表时间：
2008-10-01
影响因子：
1.8
通讯作者：
Duhaime, Ann-Christine
中科院分区：
文献类型：
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作者：
Koob, Andrew O.;Harris, Brent T.;Duhaime, Ann-Christine
由于仔猪大脑与人类大脑在解剖学和发育上的相似性，我们对描述出生后发生的细胞发生区域很感兴趣，以验证后续神经生物学研究的模型。在本研究中，4头仔猪分别于6、7和8日龄注射5-溴脱氧尿嘧啶（BrdU）。这些动物在13日龄时被处死，对其大脑进行分析，以表征细胞发生的区域。BrdU在全脑可见，在室下区（SVZ）最为丰富；双皮质素（DCX）表达细胞遍布白质，在SVZ有广泛的DCX网络。在这里，我们首次描述了使用BrdU和DCX的免疫组织化学来研究仔猪脑中的细胞发生。(c) 2008 isdn。Elsevier Ltd.出版。版权所有。
Because of the anatomical and developmental similarity of the piglet brain to the human brain we were interested in characterizing the areas of cellular genesis which occur postnatally to validate the model for subsequent neurobiological research. In this study, four piglets were injected with 5-bromodeoxyuridine (BrdU) at 6, 7 and 8 days of age. The animals were sacrificed at 13 days of age and the brains were analyzed to characterize areas of cellular genesis. BrdU was seen throughout the brain and found to be most abundant in the subventricular zone (SVZ); doublecortin (DCX) expressing cells were found throughout the white matter-with an extensive DCX network in the SVZ. Here we describe for the first time the use of immunohistochemistry for BrdU and DCX to study cellular genesis in the piglet brain. (C) 2008 ISDN. Published by Elsevier Ltd. All rights reserved.