AN ULTRABITHORAX PROTEIN BINDS SEQUENCES NEAR ITS OWN AND THE ANTENNAPEDIA P1 PROMOTERS
AN ULTRABITHORAX PROTEIN BINDS SEQUENCES NEAR ITS OWN AND THE ANTENNAPEDIA P1 PROMOTERS
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DOI：
10.1016/0092-8674(88)90251-6
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发表时间：
1988-12-23
期刊：
影响因子：
64.5
通讯作者：
HOGNESS, DS
中科院分区：
文献类型：
--
作者：
BEACHY, PA;KRASNOW, MA;HOGNESS, DS
同源异型基因Ultrabithorax（Ubx）位于果蝇的双胸复合体中，编码一个密切相关的蛋白质家族，该家族指导后胸和前腹变分粒的发育命运。我们已经从过量生产的大肠杆菌菌株中纯化了Ubx蛋白家族的一个成员，并表明它是一种序列特异性DNA结合蛋白。该蛋白质紧密结合到其自身启动子附近的序列和Antp（Antp）的P1启动子附近的序列，Antp是一种同源异型基因Ubx，从遗传研究中已知会抑制。结合位点成簇地出现在转录起始位点的下游，并且远位于Antp P1的上游。它们的大小范围为40至90 bp，并含有三核苷酸TAA或相关六核苷酸TAA-TCG的串联重复序列。这些结果表明，Ubx的调节活动是直接的，并介导的Ubx蛋白的启动子区序列的结合。
The homeotic gene Ultrabithorax (Ubx), located in the bithorax complex of Drosophila, encodes a family of closely related proteins that direct the developmental fates of posterior thoracic and anterior abdominal metameres. We have purified a member of the Ubx protein family from an overproducing Escherichia coli strain and have shown that it is a sequence-specific DNA binding protein. The protein binds tightly to sequences near its own promoter and near the P1 promoter of Antennapedia (Antp), a homeotic gene Ubx is known to repress from genetic studies. The binding sites occur in clusters downstream of the transcription start sites, and far upstream at Antp P1. They range in size from 40 to 90 bp, and contain tandem repeats of the trinucleotide TAA or the related hexanucleotide TAA-TCG. These results suggest that the regulatory activities of Ubx are direct and are mediated by binding of Ubx proteins to promoter region sequences.