Quantitation using enhanced signal tags: A technique for comparative proteomics
Quantitation using enhanced signal tags: A technique for comparative proteomics
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DOI：
10.1021/pr0255506
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发表时间：
2003-01-01
影响因子：
4.4
通讯作者：
Reilly, JP
中科院分区：
文献类型：
--
作者：
Beardsley, RL;Reilly, JP
n端和赖氨酸残基的差异氨基化为使用MALDI质谱进行蛋白质定量的新方法提供了基础。由于赖氨酸的氨基化增加了赖氨酸的碱度，从而增加了MALDI电离率，因此该方法被称为使用增强信号标签（QUEST）的定量。氨基标签不同的亚甲基，导致14个Da的质量差异。在使用MALDI-TOF质谱分析两种模型蛋白的消化过程中，QUEST的效用得到了证明。
Differential amidination of N-termini and lysine residues provides the basis for a novel approach to protein quantitation using MALDI mass spectrometry. Because the amidination of lysines increases their basicity and therefore MALDI ionization yields, the method is called quantitation using enhanced signal tags (QUEST). Amidine labels differ by methylene groups, leading to 14 Da mass differentials. The utility of QUEST is demonstrated while analyzing the digests of two model proteins using MALDI-TOF mass spectrometry.