Effects of veraguensin and galgravin on osteoclast differentiation and function
Effects of veraguensin and galgravin on osteoclast differentiation and function
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DOI：
10.1007/s10616-011-9416-z
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发表时间：
2012-05-01
期刊：
影响因子：
2.2
通讯作者：
Woo, Je-Tae
中科院分区：
文献类型：
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作者：
Asai, Midori;Lee, Ji-Won;Woo, Je-Tae
木兰属植物的干燥花蕾因其抗炎、抗疟疾和抗血小板活性而被广泛用作草药。在这里，我们发现，veraguensin和galgravin，木脂素化合物来源于木兰，剂量依赖性地抑制骨髓细胞和成骨细胞共培养物中破骨细胞的形成。这些化合物还抑制核因子κ B配体的受体激活剂（RANKL）诱导的RAW 264.7细胞和骨髓巨噬细胞中的破骨细胞分化。在RANKL诱导的信号通路中，veraguensin和galgravin减少了p38磷酸化并抑制了c-Fos的表达，c-Fos是破骨细胞生成的关键转录因子。Veraguensin和galgravin也抑制骨陷窝的形成，这是伴随着成熟的破骨细胞活力下降。总之，这些结果表明，veraguensin和galgravin可以抑制骨吸收，并可能为开发治疗骨质疏松症等骨破坏性疾病的药物提供新的化合物。
The dried flower buds of Magnolia sp. are widely used as herbal medicines because of their anti-inflammatory, anti-malarial and anti-platelet activities. Here, we found that veraguensin and galgravin, lignan compounds derived from Magnolia sp., dose-dependently inhibited osteoclast formation in co-cultures of bone marrow cells and osteoblastic cells. These compounds also inhibited receptor activator of nuclear factor kappa B ligand (RANKL)-induced osteoclast differentiation in RAW264.7 cells and bone marrow macrophages. In the RANKL-induced signaling pathway, veraguensin and galgravin reduced p38 phosphorylation and suppressed the expression of c-Fos, a key transcription factor for osteoclastogenesis. Veraguensin and galgravin also inhibited osteoclastic pit formation, which was accompanied by decreased mature osteoclast viability. In conclusion, these results indicate that veraguensin and galgravin can inhibit bone resorption and may offer novel compounds for the development of drugs to treat bone-destructive diseases such as osteoporosis.