RECENT STUDIES OF THE MINERAL PHASE IN BONE AND ITS POSSIBLE LINKAGE TO THE ORGANIC MATRIX BY PROTEIN-BOUND PHOSPHATE BONDS
RECENT STUDIES OF THE MINERAL PHASE IN BONE AND ITS POSSIBLE LINKAGE TO THE ORGANIC MATRIX BY PROTEIN-BOUND PHOSPHATE BONDS
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DOI：
10.1098/rstb.1984.0041
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发表时间：
1984-01-01
影响因子：
6.3
通讯作者：
GLIMCHER, MJ
中科院分区：
文献类型：
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作者：
GLIMCHER, MJ
解释骨矿物质的X射线衍射特征的成熟变化的最广泛接受的假设是“无定形磷酸钙理论”，该理论假设初始无定形磷酸钙固相沉积，逐渐转化为结晶度差的羟基磷灰石。本文应用X射线径向分布函数分析法和~（31）P核磁共振法对不同年龄组的骨矿物质进行了研究。已经最终证明，在任何显著的骨内不存在无定形磷酸钙的固相。31 P n.m.r.研究已经检测到在类勃氏石构型中存在酸式磷酸基团。含O-磷酸丝氨酸和O-磷酸苏氨酸的磷蛋白已从骨基质中分离出来并鉴定。组织和细胞培养已经证实，它们在骨中合成，最有可能是由成骨细胞合成。物理化学和病理生理学研究支持骨和其他脊椎动物矿化组织的无机相和有机相通过O-磷酸丝氨酸和O-磷酸苏氨酸的磷酸单酯键连接的论点，O-磷酸丝氨酸和O-磷酸苏氨酸是结构有机基质和无机磷酸钙晶体的成分。
The most widely accepted hypothesis to account for maturational changes in the X-ray diffraction characteristics of bone mineral has been the ‘amorphous calcium phosphate theory’, which postulates that an initial amorphous calcium phosphate solid phase is deposited that gradually converts to poorly crystalline hydroxyapatite. Our studies of bone mineral of different ages by X-ray radial distribution function analysis and31P n.m.r. have conclusively demonstrated that a solid phase of amorphous calcium phosphate does not exist in bone in any significant amount.31P n.m.r. studies have detected the presence of acid phosphate groups in a brushite-like configuration. Phosphoproteins containingO-phosphoserine andO-phosphothreonine have been isolated from bone matrix and characterized. Tissue and cell culture have established that they are synthesized in bone, most likely by the osteoblasts. Physicochemical and pathophysiological studies support the thesis that the mineral and organic phases of bone and other vertebrate mineralized tissues are linked by the phosphomonester bonds ofO-phosphoserine andO-phosphothreonine, which are constituents of both the structural organic matrix and the inorganic calcium phosphate crystals.