Vertical transmission of human immunodeficiency virus is correlated with the absence of high-affinity/avidity maternal antibodies to the gp120 principal neutralizing domain.
Vertical transmission of human immunodeficiency virus is correlated with the absence of high-affinity/avidity maternal antibodies to the gp120 principal neutralizing domain.
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人类免疫缺陷病毒的垂直传播与 gp120 主要中和结构域的高亲和力/亲和力母体抗体的缺乏相关。
DOI：
10.1073/pnas.87.9.3445
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发表时间：
1990
影响因子：
11.1
通讯作者：
Rubinstein,A
中科院分区：
文献类型：
--
作者：
Devash,Y;Calvelli,TA;Wood,DG;Reagan,KJ;Rubinstein,A
许多，但不是所有，感染了人类免疫缺陷病毒(HIV)的母亲所生的婴儿是在子宫中感染的。我们现在已经证明，具有针对gp120主要中和结构域(PND)的高亲和力/亲和力抗体的母亲不太可能将艾滋病毒传播给她们的孩子。描述了一种优先测量具有生物功能的、高亲和力/亲和力的抗PND抗体水平的ELISA法。在对15个母亲/新生儿血清样本的回顾研究中，该分析正确地识别了4个未感染艾滋病毒的婴儿和11个感染艾滋病毒的婴儿。其他临床和实验室参数，如p24抗原、植物血凝素有丝分裂指数和绝对表面抗原T4+细胞计数不能准确预测艾滋病毒的胎儿传播。除了介绍一种有前景的诊断工具外，这项研究还提供了体内证据，证明保护性抗体可以预防艾滋病毒感染。
Many, but not all, infants born to mothers infected with the human immunodeficiency virus (HIV) are infected in utero. We have now shown that mothers who have high-affinity/avidity antibodies directed toward the principal neutralizing domain (PND) of gp120 are less likely to transmit HIV to their children. An ELISA that preferentially measures the level of the biologically functioning, high-affinity/avidity antibodies against PND is described. In a retrospective study of 15 maternal/neonatal serum samples, the assay correctly identified the 4 uninfected and the 11 HIV-infected infants. Other clinical and laboratory parameters such as p24 antigen, phytohemagglutinin mitogenic index, and absolute surface antigen T4+ cell counts did not accurately predict HIV fetal transmission. In addition to introducing a promising diagnostic tool, this study provides the in vivo evidence that protective antibodies may prevent infection by HIV.