Red-shifted Renilla reniformis luciferase variants for imaging in living subjects
Red-shifted Renilla reniformis luciferase variants for imaging in living subjects
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DOI：
10.1038/nmeth1070
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发表时间：
2007-08-01
期刊：
影响因子：
48
通讯作者：
Gambhir, Sanjiv Sam
中科院分区：
文献类型：
--
作者：
Loening, Andreas Markus;Wu, Anna M.;Gambhir, Sanjiv Sam
利用R.肾形萤光素酶（RLuc）作为报告基因在小动物成像中受到其481 nm峰值发射光谱的阻碍，因为蓝色波长在生物组织中强烈衰减。为了克服这一点，我们产生了具有高达66 nm（547 nm峰值）的红移（红色）位移的RLuc变体，其也具有比天然RLuc更高的稳定性和更高的光发射。
The use of R. reniformis luciferase ( RLuc) as a reporter gene in small- animal imaging has been hampered by its 481 nm peaked emission spectrum, as blue wavelengths are strongly attenuated in biological tissues. To overcome this, we generated variants of RLuc with bathochromic ( red) shifts of up to 66 nm ( 547 nm peak) that also had greater stability and higher light emission than native RLuc.