Analysis of polycrystallinity in hen egg-white lysozyme using a pnCCD
Analysis of polycrystallinity in hen egg-white lysozyme using a pnCCD
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使用 pnCCD 分析鸡蛋清溶菌酶的多晶性
DOI：
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发表时间：
2012
期刊：
影响因子：
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通讯作者：
U. Pietsch
中科院分区：
文献类型：
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作者：
S. Send;A. Abboud;W. Leitenberger;M. Weiss;R. Hartmann;L. Strüder;U. Pietsch
采用pnCCD探测器，利用白色同步辐射能量色散X射线劳厄衍射技术，对鸡蛋-白色溶菌酶晶体进行了分析。从任意取向的晶体的单次曝光中测量的劳厄斑点，可以以约2.5%的准确度提取四元晶胞的晶格常数。扫描整个样品表面，在不同位置记录具有分裂反射的劳厄图像。相应的衍射图案由两个结晶域产生，相对于彼此具有约1°的倾斜。所获得的结果表明，pnCCD的快速X射线筛选的晶体的大分子或蛋白质之前，常规的X射线结构分析的潜力。所描述的实验可以自动化以定量表征不完美的单晶或多晶。
A crystal of hen egg-white lysozyme was analyzed by means of energy-dispersive X-ray Laue diffraction with white synchrotron radiation at 2.7 A resolution using a pnCCD detector. From Laue spots measured in a single exposure of the arbitrarily oriented crystal, the lattice constants of the tetragonal unit cell could be extracted with an accuracy of about 2.5%. Scanning across the sample surface, Laue images with split reflections were recorded at various positions. The corresponding diffraction patterns were generated by two crystalline domains with a tilt of about 1° relative to each other. The obtained results demonstrate the potential of the pnCCD for fast X-ray screening of crystals of macromolecules or proteins prior to conventional X-ray structure analysis. The described experiment can be automatized to quantitatively characterize imperfect single crystals or polycrystals.