Association of vasopressin 1a receptor levels with a regulatory microsatellite and behavior
Association of vasopressin 1a receptor levels with a regulatory microsatellite and behavior
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DOI：
10.1111/j.1601-183x.2005.00119.x
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发表时间：
2005-07-01
影响因子：
2.5
通讯作者：
Young, LJ
中科院分区：
文献类型：
--
作者：
Hammock, EAD;Lim, MM;Young, LJ
加压素以物种特异性的方式调节复杂的行为，如焦虑、养育、社会参与、依恋和攻击。加压素调节这些行为的能力被认为取决于加压素1a受体（V1aRs）在脑中的物种特异性分布模式。有相当大的个体差异的V1aR结合在草原田鼠物种，田鼠，ochrogaster的大脑中的模式。我们推测，这种V1aR表达水平的个体差异与草原田鼠v1ar基因5'调控区多态性微卫星的个体差异有关。此外，我们假设V1aR表达的个体差异有助于加压素依赖性行为的个体差异。为了验证这些假设，我们首先使用测量焦虑相关行为和社会行为的测试筛选了20只成年雄性草原田鼠的行为变异。然后，我们用受体放射自显影术评估了这些动物大脑的V1aR变异性，并使用聚合酶链反应对相同动物的v1ar基因5'微卫星多态性长度进行基因分型。在这份报告中，我们描述了这种基于发现的实验方法的结果，以确定潜在的基因，大脑和行为的相互关系。分析表明，在某些但不是所有的大脑区域中，V1aR水平与微卫星长度相关，并且这些和其他大脑区域中的V1aR水平与焦虑相关和社会行为相关。这些结果产生了新的假设，关于神经控制焦虑相关的和社会行为，并产生洞察的潜在机制，非编码基因多态性可能会影响行为特征。
Vasopressin regulates complex behaviors such as anxiety, parenting, social engagement and attachment and aggression in a species-specific manner. The capacity of vasopressin to modulate these behaviors is thought to depend on the species-specific distribution patterns of vasopressin la receptors (V1aRs) in the brain. There is considerable individual variation in the pattern of V1aR binding in the brains of the prairie vole species, Microtus; ochrogaster. We hypothesize that this individual variability in V1aR expression levels is associated with individual variation in a polymorphic microsatellite in the 5' regulatory region of the prairie vole v1ar gene. Additionally, we hypothesize that individual variation in V1aR expression contributes to individual variation in vasopressin-dependent behaviors. To test these hypotheses, we first screened 20 adult male prairie voles for behavioral variation using tests that measure anxiety-related and social behaviors. We then assessed the brains of those animals for V1aR variability with receptor autoradiography and used polymerase chain reaction to genotype the same animals for the length of their 5' microsatellite polymorphism in the v1ar gene. In this report, we describe the results of this discovery-based experimental approach to identify potential gene, brain and behavior interrelationships. The analysis reveals that V1aR levels, in some but not all brain regions, are associated with microsatellite length and that V1aR levels in those and other brain regions correlate with anxiety-related and social behaviors. These results generate novel hypotheses regarding neural control of anxiety-related and social behaviors and yield insight into potential mechanisms by which non-coding gene polymorphisms may influence behavioral traits.