Clinical effect of T-SPOT.TB test for the diagnosis of tuberculosis
Clinical effect of T-SPOT.TB test for the diagnosis of tuberculosis
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DOI：
10.1186/s12879-019-4597-8
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发表时间：
2019-11-21
影响因子：
3.7
通讯作者：
Wang, Xiaoqin
中科院分区：
文献类型：
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作者：
Ma, Yanfen;Li, Ruicheng;Wang, Xiaoqin
本研究的目的是进一步研究T-SPOT. TB检测在诊断结核病（TB）中的临床有效性，包括T-SPOT. TB检测对评估不同类型和部位的TB的影响。方法收集20,332例疑似结核病患者的标本。然后对T-SPOT. TB结果与临床诊断进行综合分析，评价不同年龄组、样本类型和医院科室T-SPOT. TB检测的构成比和阳性检出率。此外，我们还比较了潜伏性结核感染（LTBI）、活动性结核感染和陈旧性结核感染组的斑点数量和构成比。将活动性肺结核组进一步分为肺结核（PTB）、肺及肺外结核（PETB）和肺外结核（EPTB）亚组，评价各亚组间结核菌斑数和构成比是否有统计学差异。结果不同年龄组、不同标本类型和不同科室的结核病患者均出现T-SPOT. TB试验阳性结果。老年患者组、胸腔积液样本和胸外科显示阳性率最高。CFP-10和ESAT-6威尔斯孔的斑点数在疾病组或活动性TB亚组之间没有统计学显著差异。然而，当ESAT-6和CFP-10威尔斯孔为双阳性时，组成率显著不同。结核菌斑数和构成比在3个疾病组间差异有统计学意义，而在3个活动性结核亚组间差异无统计学意义。结论T-SPOT结果。TB试验显示LTBI、活动性TB和陈旧性TB存在差异。此外，在活动性TB组中观察到更高的斑点数量水平。
Background The goal of this study was to further investigate the clinical effectiveness of the T-SPOT.TB test in diagnosing tuberculosis (TB), including the effects of T-SPOT.TB test on evaluating diverse TB types and locations. Methods We collected 20,332 specimens from patients suspected to have TB. Afterwards, we performed an integrative analysis of T-SPOT.TB results and clinical diagnoses, and evaluated the composition ratio and positive detection rate of the T-SPOT.TB test in various age groups, sample types, and hospital departments. In addition, we compared the spot number and composition rate between latent TB infection (LTBI), active TB infection, and old TB infection groups. The active TB group was then further divided into pulmonary TB (PTB), pulmonary and extrapulmonary TB (PETB), and extrapulmonary TB (EPTB) subgroups, and we evaluated whether there were statistical differences in spot number and composition rate between subgroups. Results Positive results from the T-SPOT.TB test were found across different age groups, specimen types, and hospital departments. Elderly patient groups, pleural effusion samples, and thoracic surgery departments showed the highest rates of positivity. There were no statistically significant differences in spot number of CFP-10 and ESAT-6 wells between disease groups or active TB subgroups. The composition rate, however, was significantly different when ESAT-6 and CFP-10 wells were double-positive. The spot number and composition rate were statistically different between the three disease groups, but showed no significant differences between the three subgroups of active TB. Conclusions The results of T-SPOT. TB test showed differences in LTBI, active TB and old TB. Additionally, a higher spot number level was observed in the active TB group.