Reproductive cycle and biochemical composition of the Zhe oyster Crassostrea plicatula Gmelin in an eastern coastal bay of China
Reproductive cycle and biochemical composition of the Zhe oyster Crassostrea plicatula Gmelin in an eastern coastal bay of China
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DOI：
10.1016/j.aquaculture.2006.08.023
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发表时间：
2006-11
期刊：
影响因子：
4.5
通讯作者：
Qi Li;Wenguang Liu;Kunio Shirasu;Weimin Chen;Shouxuan Jiang
中科院分区：
文献类型：
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作者：
Qi Li;Wenguang Liu;Kunio Shirasu;Weimin Chen;Shouxuan Jiang
2004年3月至2005年2月，对乳山湾褶牡蛎（Crassostrea plicatula）的繁殖周期和生化组成进行了研究，并分析了温度、盐度和叶绿素a等环境因子对褶牡蛎繁殖周期和生化组成的影响。组织学分析，结合卵母细胞检查和测量蛋白质，糖原和脂质水平和RNA/DNA比值从性腺，闭壳肌和外套膜组织进行。对C.褶皱发育包括两个阶段：一个休息期（11月至2月）和繁殖活跃期，包括成熟和产卵，在一年的其余时间。春季和夏季的配子发生与卵巢中蛋白质和脂质含量的增加有关，并以牺牲先前在冬季积累的储备（主要是糖原）为代价。RNA/DNA比值是卵巢性成熟的良好指标;卵巢中RNA/DNA比值的增加似乎表明卵巢内卵黄蛋白合成活性的增加。产卵发生的时刻，水温和叶绿素a水平最高，盐度最低的全年。在储存和配子发育过程中糖原含量的高变化表明，碳水化合物是该物种的主要呼吸底物。结果表明，C.褶瓣花在配子发生模式上可能是一个保守的种。
The reproductive cycle and biochemical composition of the oyster Crassostrea plicatula in Rushan Bay, an eastern coastal bay of China, were investigated between March 2004 and February 2005 in relation to environmental factors (temperature, salinity and chlorophyll a). Histological analysis, combined with oocyte examination and measurements of protein, glycogen and lipid levels and RNA/DNA ratio from gonad, adductor muscle and mantle tissue of each sex were performed. The gametogenic cycle of C. plicatula comprised two phases: a resting phase (November–February) and reproductively active phase, including ripeness and spawning, during the rest of year. Gametogenesis in spring and summer was associated with an increase in protein and lipid contents in the ovaries, and took place at the expense of reserves (mainly glycogen) accumulated previously during winter. The RNA/DNA ratio is a good indicator of sexual maturity in the ovary; the increasing RNA/DNA ratio in the ovary appears to show the rising synthetic activity of vitellin within the ovary. Spawning occurred at the moment when water temperature and chlorophyll a levels were highest, and salinity lowest throughout the year. High variation in glycogen contents during storage and gametogenic development suggests that carbohydrates are the main respiratory substrate in this species. The results demonstrated that C. plicatula may be considered a conservative species in gametogenic pattern.