Nucleotide sequence of the human parathyroid hormone gene.
Nucleotide sequence of the human parathyroid hormone gene.
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DOI：
10.1073/pnas.80.8.2127
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发表时间：
1983-04
影响因子：
11.1
通讯作者：
T. Vasicek;B. E. McDevitt;M. Freeman;B. Fennick;G. Hendy;J. Potts;A. Rich;H. Kronenberg
中科院分区：
文献类型：
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作者：
T. Vasicek;B. E. McDevitt;M. Freeman;B. Fennick;G. Hendy;J. Potts;A. Rich;H. Kronenberg
我们从 lambda 噬菌体基因库中分离出含有编码人前甲状旁腺激素原的基因的噬菌体。通过使用噬菌斑杂交技术和体内重组选择技术来分离噬菌体。人前甲状旁腺激素原基因包含两个间插序列，将基因分为5'非编码结构域、“前原”序列结构域以及包含甲状旁腺激素序列和3'非编码区的结构域。该基因大约有 4,200 个碱基对长。人类白细胞 DNA 的限制性内切核酸酶分析表明，单倍体人类基因组含有一份前甲状旁腺激素原基因。具有采用 Z-DNA 构象潜力的交替嘌呤和嘧啶的 14 碱基对序列位于假定的转录起始位点上游 134 碱基对处。
From a lambda phage gene library we have isolated phage containing the gene encoding human preproparathyroid hormone. The phage were isolated by using both the plaque-hybridization technique and the in vivo recombination-selection technique. The human preproparathyroid hormone gene contains two intervening sequences that separate the gene into a 5' noncoding domain, a "prepro" sequence domain, and a domain containing the parathyroid hormone sequence and the 3' noncoding region. The gene is approximately 4,200 base pairs long. Restriction endonuclease analysis of human leukocyte DNA shows that the haploid human genome contains one copy of the preproparathyroid hormone gene. A 14-base-pair sequence of alternating purines and pyrimidines that has the potential of adopting the Z-DNA conformation lies 134 base pairs upstream from the presumed site of initiation of transcription.