Proteomic identification of radiation response markers in mouse intestine and brain
Proteomic identification of radiation response markers in mouse intestine and brain
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DOI：
10.1002/pmic.201000332
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发表时间：
2011-04-01
期刊：
影响因子：
3.4
通讯作者：
Lee, Yun-Sil
中科院分区：
文献类型：
--
作者：
Lim, Young-Bin;Pyun, Bo-Jeong;Lee, Yun-Sil
目前正在加紧努力，在基因组和蛋白质组学水平上发展更敏感和更快的分子方法，以鉴定电离辐射照射后的蛋白质标记物。然而，很少有特异性的蛋白质标记物，特别是器官特异性标记物被鉴定出来。在本研究中，我们采用2-DE分析方法分析了1只gy照射小鼠脑、肺、脾和肠各组织中蛋白表达的变化。MALDI-TOF质谱和肽图谱鉴定出25个表达变化大于2倍的蛋白。为了确认对照和红外处理样品之间的显著差异，选择10个具有可用商业抗体的鉴定蛋白进行免疫印迹。其中，只有5个显示出与2-DE数据相似的蛋白质表达模式。分别是热休克蛋白5 （hsp5）、hsp90kda β、hsp1、转醛缩酶1 （TA1）和磷酸甘油酸激酶1 （PGK1）。其中，PGK1在小鼠肠道中特异性上调，TA1在小鼠脑中特异性下调。TA1在其他组织中的表达未见改变。基于这些数据，我们认为TA1和PGK1可以被认为是IR暴露的候选组织特异性蛋白标志物。
Increasing efforts are being made to develop more sensitive and faster molecular methodologies at the genomic and proteomic levels for the identification of protein markers after exposure to ionizing radiation (IR). However, few specific protein markers, especially organ-specific markers, have been identified. In this study, we analyzed altered protein expressions in various tissues, namely, brain, lung, spleen, and intestine, from 1 Gy-irradiated mice by employing 2-DE analysis. MALDI-TOF MS and peptide mapping identified 25 proteins that showed greater than twofold expressional changes. In order to confirm significant differences between control and IR-treated samples, ten identified proteins with available commercial antibodies were selected for immunoblotting. Of these, only five showed protein expression patterns that were similar to 2-DE data. These were heat shock protein 5 (HSP 5), HSP 90 kDa beta, HSP 1, transaldolase 1 (TA1), and phosphoglycerate kinase 1 (PGK1). In particular, PGK1 was specifically upregulated in mouse intestine, and TA1 was specifically downregulated in brain by irradiation. TA1 expression was unaltered in other tissues. Based on these data, we suggest that TA1 and PGK1 can be considered as candidate tissue-specific protein markers of IR exposure.