Genetic and phylogenetic analyses of capsid protein gene in feline calicivirus isolates from Rio Grande do Sul in southern Brazil
Genetic and phylogenetic analyses of capsid protein gene in feline calicivirus isolates from Rio Grande do Sul in southern Brazil
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DOI：
10.1016/j.virusres.2011.12.008
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发表时间：
2012-02-01
期刊：
影响因子：
5
通讯作者：
Weiblen, R.
中科院分区：
文献类型：
--
作者：
Henzel, A.;Sa e Silva, M.;Weiblen, R.
猫杯状病毒(FCV)是影响家猫的一种重要病原体，可引起急性口腔和上呼吸道症状。本研究的目的是分析巴西南部不同FCV分离株衣壳蛋白的变异性。通过检查开放阅读框架2(ORF2，B-F区)，对13个巴西FCV样本进行了测序分析，对之前发表的10个序列进行了系统发育分析和评估。巴西FCV株与FCV-F9株ORF2的预测氨基酸序列比较表明，主要差异位于C区和高变区E(Hvr_E)。与菌株F9相比，定位于D区、5‘HvR_E区和保守E区的表位也表现出一定的变异性。这是首次描述巴西口蹄疫病毒分离株之间的序列分析和系统发育关系的研究。本文提出的结果可能会扩展目前关于FCV生物学、流行病学和遗传多样性方面的知识，并为改进当前FCV疫苗的有效性提供见解。(C)2011爱思唯尔B.V.保留所有权利。
Feline calicivirus (FCV) is an important pathogen that affects domestic cats, inducing acute oral and upper respiratory tract clinical signs. The aim of this study was to analyze the variability of the capsid protein in different FCV isolates from southern Brazil. The sequencing analyses of thirteen Brazilian FCV samples, phylogenetic analyses and assessments of ten previously published sequences were conducted by examining the open reading frame 2 (ORF2, regions B-F). Comparisons of the predicted amino acid sequences of the ORF2 in Brazilian FCV isolates with those of the FCV-F9 strain indicated that the main differences are located within the regions C and hypervariable E (HVR_E). Epitopes that were mapped to the regions D, 5'HVR_E and conserved E also presented with some variability when compared to the strain F9. This is the first study describing sequence analyses and the phylogenetic relationships among FCV isolates from Brazil. The results presented here may expand upon current knowledge regarding aspects of FCV biology, epidemiology and genetic diversity and provide insights into improving the efficacies of current FCV vaccines. (C) 2011 Elsevier B.V. All rights reserved.