METHOD FOR ISOLATION OF NONESTERIFIED FATTY-ACIDS AND SEVERAL OTHER CLASSES OF PLASMA-LIPIDS BY COLUMN CHROMATOGRAPHY ON SILICA-GEL
METHOD FOR ISOLATION OF NONESTERIFIED FATTY-ACIDS AND SEVERAL OTHER CLASSES OF PLASMA-LIPIDS BY COLUMN CHROMATOGRAPHY ON SILICA-GEL
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DOI：
10.1016/0378-4347(93)80441-6
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发表时间：
1993-09-08
期刊：
影响因子：
--
通讯作者：
HOPPEL, CL
中科院分区：
文献类型：
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作者：
INGALLS, ST;KRIARIS, MS;HOPPEL, CL
描述了一种通过萃取和硅胶柱色谱法从人血浆中分离非酯化脂肪酸、胆固醇酯、甘油三酯、胆固醇和甘油二酯、甘油单酯和一些磷脂的方法。除甘油二酯和胆固醇外，所有这些脂质类在七个洗脱步骤中完全分离。将甘油二酯和胆固醇一起分离。在柱色谱步骤中，代表最显著的血浆脂质类别的模型化合物几乎完全回收。在两个样品分离步骤后，血浆样本中添加的十七烷酸的总回收率为81%。在两个样品制备步骤后，添加的合成十五烷酸和十七烷酸酯脂质同系物从血浆中的总回收率为80 - 91%。从20份血浆样本中提取和分离这些脂质类（一式两份）需要约6 h。或者，通过完整程序的变化，可以在4小时内从20份血浆标本中一式两份分离非酯化脂肪酸。
A method is described for isolation from human plasma of non-esterified fatty acids, cholesteryl esters, triglycerides, cholesterol and diglycerides, monoglycerides, and some phospholipids by extraction and silica gel column chromatography. All of these lipid classes except diglycerides and cholesterol were separated cleanly in seven elution steps. Diglycerides and cholesterol were isolated together. Recovery of model compounds which represent the most significant classes of plasma lipids during the column chromatographic step was nearly complete. The overall recovery of added heptadecanoic acid from plasma specimens was 81% after both sample isolation steps. The overall recovery of added synthetic pentadecanoic acid and heptadecanoic acid ester lipid homologues from plasma was 80-91% after both sample preparation steps. About 6 h are required for extraction and isolation in duplicate of these lipid classes from twenty plasma specimens. Alternatively, non-esterified fatty acids can be isolated from twenty plasma specimens in duplicate within 4 h by a variation of the full procedure.