A genome-wide set of congenic mouse strains derived from DBA/2J on a C57BL/6J background
A genome-wide set of congenic mouse strains derived from DBA/2J on a C57BL/6J background
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DOI：
10.1016/j.ygeno.2005.05.010
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发表时间：
2005-09-01
期刊：
影响因子：
4.4
通讯作者：
Lusis, AJ
中科院分区：
文献类型：
--
作者：
Davis, RC;Schadt, EE;Lusis, AJ
在复杂性状的分析中，同源菌株是强有力的工具，因为它们允许在整个基因组的其余部分没有遗传变异的情况下对单个位点进行表征。在这里，我们报告了从供体菌株DBA/2J衍生的背景菌株C57BL/6J的全基因组同源菌株的构建和初步鉴定。对于许多菌株，我们进行了高密度SNP基因分型，以精确绘制基因间隔并确定任何污染区域。某些品系在产仔数和雌雄比例上表现出惊人的变化。我们通过“门德尔化”心肌钙化的复杂特征来说明该集合的实用性。这65个菌株覆盖了95%以上的常染色体基因组，有助于分析这些亲本菌株之间的许多遗传性状差异。(c) 2005爱思唯尔公司版权所有。
In the analysis of complex traits, congenic strains are powerful tools because they allow characterization of a single locus in the absence of genetic variation throughout the remainder of the genome. Here, we report the construction and initial characterization of a genome-wide panel of congenic strains derived from the donor strain DBA/2J on the background strain C57BL/6J. For many strains, we have carried out high-density SNP genotyping to precisely map the congenic interval and to identify any contaminating regions. Certain strains exhibit striking variation in litter size and in the ratio of females to males. We illustrate the utility of the set by "Mendelizing" the complex trait of myocardial calcification. These 65 strains cover more than 95% of the autosomal genome and should facilitate the analysis of the many genetic trait differences that have been reported between these parental strains. (c) 2005 Elsevier Inc. All rights reserved.