Mitofusin 2 is involved in chemotaxis of neutrophil-like differentiated HL-60 cells
Mitofusin 2 is involved in chemotaxis of neutrophil-like differentiated HL-60 cells
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DOI：
10.1016/j.bbrc.2019.04.037
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发表时间：
2019-06-04
影响因子：
3.1
通讯作者：
Sabe, Hisataka
中科院分区：
文献类型：
--
作者：
Mazaki, Yuichi;Takada, Shingo;Sabe, Hisataka
中性粒细胞在识别入侵者后迅速迁移到感染部位。在趋化过程中，中性粒细胞需要线粒体氧化磷酸化（OXPHOS）提供的能量，而中性粒细胞在正常条件下严重依赖糖酵解。线粒体OXPHOS与线粒体形态学相关。在这里，我们研究了嗜中性粒细胞样分化的HL-60细胞的线粒体形态后，化学引诱剂N-甲酰-蛋氨酸-亮氨酸-苯丙氨酸（fMLP）刺激。我们发现，线粒体形态变化的管状fMLP刺激后。线粒体OXPHOS活性和线粒体复合物II在fMLP刺激后显著增加。另一方面，线粒体融合蛋白线粒体融合蛋白2（MFN 2）的沉默抑制线粒体形态学的变化。此外，MFN 2沉默抑制OXPHOS激活和fMLP刺激后的趋化性。这些结果表明，MFN 2参与了依赖于线粒体的分化HL-60细胞的趋化性。(C)2019爱思唯尔公司All rights reserved.
Neutrophils rapidly migrate to infection sites after the recognition of invaders. During chemotaxis, neutrophils require energy supplied by mitochondria oxidative phosphorylation (OXPHOS), whereas neutrophils rely heavily on glycolysis under normal conditions. Mitochondrial OXPHOS correlates with mitochondrial morphology. Here, we examined the mitochondrial morphology of neutrophil-like differentiated HL-60 cells after chemoattractant N-formyl-Met-Leu-Phe (fMLP) stimulation. We found that mitochondrial morphology changes to a tubular form after fMLP stimulation. Mitochondrial OXPHOS activity and mitochondria, complex II significantly increased after fMLP stimulation. On the other hand, the silencing of mitochondrial fusion protein mitofusin 2 (MFN2) suppresses mitochondrial morphological changes. Furthermore, MFN2 silencing suppressed OXPHOS activation and chemotaxis after fMLP stimulation. These results suggest that MFN2 is involved in chemotaxis of differentiated HL-60 cells depending on mitochondria. (C) 2019 Elsevier Inc. All rights reserved.