Gene Expression Profiling of Valvular Interstitial Cells in Rapacz Familial Hypercholesterolemic Swine.
Gene Expression Profiling of Valvular Interstitial Cells in Rapacz Familial Hypercholesterolemic Swine.
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DOI：
10.1016/j.gdata.2014.08.004
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发表时间：
2014-12-01
期刊：
影响因子：
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通讯作者：
Masters, Kristyn S
中科院分区：
文献类型：
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作者：
Porras, Ana M;Shanmuganayagam, Dhanansayan;Meudt, Jennifer J;Krueger, Christian G;Reed, Jess D;Masters, Kristyn S
Rapacz家族性高胆固醇血症(RFH)猪是一种公认的人类FH模型，FH是一种高度常见的遗传性疾病，与冠状动脉疾病和钙化性主动脉瓣疾病(CAVD)的风险增加有关。然而，虽然这些动物已被广泛用于动脉粥样硬化的研究，但RFH猪的心脏瓣膜以前从未被检查过。本文报道了利用Affymetrix猪基因组芯片(GEO#：GSE53997)对成年(2岁)和幼年(3月龄)RFH和WT猪心脏瓣膜间质细胞基因表达的分析。主成分和系统聚类分析表明，RFH幼鱼组和WT幼鱼组之间存在分组，几乎没有变异。此外，只有21个基因在这两个试验组之间差异表达，而当比较RFH或WT幼猪与RFH成猪时，有900多个基因差异表达。
Rapacz familial hypercholesterolemic (RFH) swine is a well-established model of human FH, a highly prevalent hereditary disease associated with increased risk of coronary artery disease and calcific aortic valve disease (CAVD). However, while these animals have been used extensively for the study of atherosclerosis, the heart valves from RFH swine have not previously been examined. We report the analysis of valvular interstitial cell gene expression in adult (two year old) and juvenile (three months old) RFH and WT swine by microarray analysis via the Affymetrix Porcine Genome Array (GEO #: GSE53997). Principal component and hierarchical clustering analysis revealed grouping and almost no variability between the RFH juvenile and WT juvenile groups. Additionally, only 21 genes were found differentially expressed between these two experimental groups whereas over 900 genes were differentially expressed when comparing either RFH or WT juvenile swine to RFH adults.