Colocalization of dopamine and GABA in spinal cord neurones in the sea lamprey
Colocalization of dopamine and GABA in spinal cord neurones in the sea lamprey
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DOI：
10.1016/j.brainresbull.2007.10.062
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发表时间：
2008-05-15
影响因子：
3.8
通讯作者：
Anadon, Ramon
中科院分区：
文献类型：
--
作者：
Celina Rodicio, M.;Villar-Cervino, Verona;Anadon, Ramon
本研究采用免疫荧光双标记法研究了上游洄游成年七鳃鳗（Petromyzonmarinus）嘴侧脊髓神经元中多巴胺（DA）和GABA神经递质的共定位。免疫荧光双标记显示，所有的DA免疫反应阳性（ir）接触脑脊液（CSF-c）细胞、近30%的中腹侧DA免疫反应阳性细胞和位于中央管外侧的大部分DA免疫反应阳性细胞也是GABA免疫反应阳性细胞，同时还发现一些DA免疫反应阳性细胞位于中央管的背侧，这增加了七鳃鳗体内已知的多巴胺能细胞类型。双标纤维主要分布在腹柱，双标终末与背侧部分GABA-ir CSF-c细胞、非CSF-c细胞和运动神经元腹内侧树突接触。这些发现揭示了多巴胺和GABA在某些细胞和纤维中的共定位，这表明这些物质在某些突触末梢中共同释放。虽然多巴胺能/GABA能CSF-c细胞在其他脊椎动物中也有报道，但其他双标记的脊髓细胞群似乎仅限于七鳃鳗。(C)2007爱思唯尔公司All rights reserved.
In this study, double immunofluorescence methods were used to investigate possible colocalization of the neurotransmitters dopamine [DA] and GABA in rostra] spinal cord neurones in the upstream migrating adult sea lamprey (Petromyzon marinus). Double immunofluorescence revealed that all the DA-immunoreactive (ir) cerebrospinal fluid-contacting (CSF-c) cells, approximately 30% of the medioventral DA-ir cells, and most of the DA-ir cells located in the grey lateral to the central canal were also GABA-ir. The results also revealed some DA-ir cells located dorsally to the central canal, which increases the number of dopaminergic cell types known in lamprey. Double-labelled fibres were mainly distributed in the ventral column, and double-labelled boutons contacted some dorsal GABA-ir CSF-c cells, as well as some non-CSF-c GABA-ir cells and ventromedial dendrites of motoneurones. The findings reveal colocalization of dopamine and GABA in some cells and fibres, which suggests co-release of these substances in some synaptic terminals. Although dopaminergic/GABAergic CSF-c cells have been reported in some other vertebrates, the other double-labelled spinal populations appear exclusive to lampreys. (C) 2007 Elsevier Inc. All rights reserved.