Inflammation-induced lymphangiogenesis in the cornea arises from CD11 b-positive macrophages
Inflammation-induced lymphangiogenesis in the cornea arises from CD11 b-positive macrophages
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DOI：
10.1172/jci23874
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发表时间：
2005-09-01
影响因子：
15.9
通讯作者：
Streilein, JW
中科院分区：
文献类型：
--
作者：
Maruyama, K;Li, M;Streilein, JW
在发炎的角膜中，血液和淋巴管平行地长入正常无血管的角膜。我们测试了适应性和/或先天性免疫细胞是否积极参与新淋巴管的发生。我们的研究结果表明，先天性免疫细胞（CD 11b（+）巨噬细胞，而不是CD 11 c（+）树突状细胞）物理上有助于淋巴管生成的病理条件下，骨髓来源的CD 11b（+）巨噬细胞表达淋巴管内皮标志物，如LYVE-1和Prox-1在小鼠角膜基质炎症条件下。此外，表达Tie 2启动子的血管内皮细胞在炎症条件下对新形成的淋巴管没有贡献。我们的体外实验表明，CD 11b（+）巨噬细胞单独能够形成表达淋巴管内皮标记物（如LYVE-1和podoplanin）的管状结构。CD 11b（+）巨噬细胞对于眼部炎症依赖性淋巴管生成的发展至关重要，这一新发现提示了淋巴管生成的新机制。
In the inflamed cornea, there is a parallel outgrowth of blood and lymphatic vessels into the normally avascular cornea. We tested whether adaptive and/or innate immune cells were actively involved in the genesis of new lymphatic vessels. Our results indicate that innate immune cells (CD11b(+) macrophages, but not CD11c(+) dendritic cells) physically contributed to lymphangiogenesis under pathological conditions and that bone marrow-derived CD11b(+) macrophages expressed lymphatic endothelial markers such as LYVE-1 and Prox-1 under inflamed conditions in the corneal stromata of mice. Furthermore, blood vascular endothelial cells that expressed the Tie2 promoter did not contribute to newly formed lymphatic vessels under inflamed conditions. Our in vitro experiments demonstrated that CD11b(+) macrophages alone were capable of forming tube-like structures that expressed markers of lymphatic endothelium such as LYVE-1 and podoplanin. The novel finding that CD11b(+) macrophages are critical for the development of inflammation-dependent lymphangiogenesis in the eye suggests a new mechanism of lymphangiogenesis.