Determination of cellular strains by combined atomic force microscopy and finite element modeling
Determination of cellular strains by combined atomic force microscopy and finite element modeling
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DOI：
10.1016/s0006-3495(02)75214-4
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发表时间：
2002-08-01
影响因子：
3.4
通讯作者：
Horton, MA
中科院分区：
文献类型：
--
作者：
Charras, GT;Horton, MA
许多器官由于生理变化或疾病而适应其机械环境。细胞既是这个过程的探测器，也是这个过程的效应器。虽然已经在体外进行了许多研究，以研究检测和适应机械应变的机制，但细胞应变仍然未知，并且无法比较不同刺激技术的结果。通过结合原子力显微镜与有限元建模和计算流体动力学的细胞轮廓和弹性的实验测定，我们报告了常见的全细胞应变技术和显微操作技术所施加的细胞应变分布，从而使它们的比较。使用我们自己的分析和其他人进行的实验数据，我们研究了不同信号转导过程的激活阈值和它们可能检测到的应变成分。我们发现，通过增加细胞骨架的F-肌动蛋白含量或细胞泊松比来调节细胞弹性是抵抗流体剪切或静水压力的良好策略。我们报告说，杂散流体流动在一些基板拉伸系统eltered显着的细胞株。总之，这项技术在进一步了解机械力，应变检测，基因表达和生理学和疾病中的细胞适应之间的相互作用方面显示出了希望。
Many organs adapt to their mechanical environment as a result of physiological change or disease. Cells are both the detectors and effectors of this process. Though many studies have been performed in vitro to investigate the mechanisms of detection and adaptation to mechanical strains, the cellular strains remain unknown and results from different stimulation techniques cannot be compared. By combining experimental determination of cell profiles and elasticities by atomic force microscopy with finite element modeling and computational fluid dynamics, we report the cellular strain distributions exerted by common whole-cell straining techniques and from micromanipulation techniques, hence enabling their comparison. Using data from our own analyses and experiments performed by others, we examine the threshold of activation for different signal transduction processes and the strain components that they may detect. We show that modulating cell elasticity, by increasing the F-actin content of the cytoskeleton, or cellular Poisson ratio are good strategies to resist fluid shear or hydrostatic pressure. We report that stray fluid flow in some substrate-stretch systems elicits significant cellular strains. In conclusion, this technique shows promise in furthering our understanding of the interplay among mechanical forces, strain detection, gene expression, and cellular adaptation in physiology and disease.