Molecular Identification, Mycotoxin Production and Comparative Pathogenicity of Fusarium temperatum Isolated from Maize in China
Molecular Identification, Mycotoxin Production and Comparative Pathogenicity of Fusarium temperatum Isolated from Maize in China
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DOI：
10.1111/jph.12164
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发表时间：
2014-03-01
影响因子：
1.5
通讯作者：
Liao, Yu-Cai
中科院分区：
文献类型：
--
作者：
Wang, Jian-Hua;Zhang, Jing-Bo;Liao, Yu-Cai
最近从比利时玉米中分离的镰刀菌种群被鉴定为一个新种，Fusarium temperatum。通过对2009年我国19个省（市、自治区）玉米穗腐病镰刀菌的调查，从贵州和湖北分离到10株镰刀菌，经鉴定为温度镰刀菌（F. temperature）。基于单个翻译延伸因子1-α和微管蛋白基因的DNA序列的形态学和分子系统发育分析显示，回收的分离物产生典型的具有足状基细胞的四隔大分生孢子，并且属于F.temperatum，其与玉米中最接近的种F. subarachanans和其他Gibberella fujikuroi复合种中的其他种明显不同。该菌株在田间均能侵染玉米和小麦，且对玉米的致病力较强。采用超高效液相色谱-串联质谱和气相色谱-质谱联用技术对田间自然条件下玉米籽粒中的真菌毒素进行了检测，结果表明，在15种真菌毒素中，检测到伏马菌素B-1和B-2两种，含量范围为9.26 ~ 166.89 g/g，FB 2真菌毒素比FB 1多得多（2.8- 108.8倍）。该真菌毒素产生谱不同于比利时人群，其中仅伏马菌素B-1在测定的11个菌株中的一个中几乎未检测到。对温镰孢菌和枯草镰孢菌菌株的比较分析表明，温镰孢菌群体中伏马菌素产生量最高，这也是对玉米致病性最强的群体。这些结果表明，需要适当的监测和控制该物种在相关的玉米种植区。
A recently isolated Fusarium population from maize in Belgium was identified as a new species, Fusarium temperatum. From a survey of Fusarium species associated with maize ear rot in nineteen provinces in 2009 in China, ten strains isolated from Guizhou and Hubei provinces were identified as F.temperatum. Morphological and molecular phylogenetic analyses based on the DNA sequences of individual translation elongation factor 1-alpha and -tubulin genes revealed that the recovered isolates produced macroconidia typical of four-septate with a foot-shaped basal cell and belonged to F.temperatum that is distinctly different from its most closely related species F.subglutinans and others within Gibberella fujikuroi complex species from maize. All the strains from this newly isolated species were able to infect maize and wheat in field, with higher pathogenicity on maize. Mycotoxin determination of maize grains infected by the strains under natural field condition by ultra-high-performance liquid chromatography-tandem mass spectrometry and gas chromatography-mass spectrometry analyses showed that among fifteen mycotoxins assayed, two mycotoxins fumonisin B-1 and B-2 ranging from 9.26 to 166.89g/g were detected, with massively more FB2 mycotoxin (2.8- to 108.8-fold) than FB1. This mycotoxin production profile is different from that of the Belgian population in which only fumonisin B-1 was barely detected in one of eleven strains assayed. Comparative analyses of the F.temperatum and F.subglutinans strains showed that the highest fumonisin producers were present among the F.temperatum population, which were also the most pathogenic to maize. These results suggested a need for proper monitoring and controlling this species in the relevant maize-growing regions.