Mechanical properties of acellular peripheral nerve
Mechanical properties of acellular peripheral nerve
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DOI：
10.1016/s0022-4804(03)00255-5
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发表时间：
2003-10-01
影响因子：
2.2
通讯作者：
Dennis, RG
中科院分区：
文献类型：
--
作者：
Borschel, GH;Kia, KF;Dennis, RG
背景脱细胞神经已被用于实验模型作为周围神经替代物。我们的目的是确定新鲜和化学处理的脱细胞周围神经之间的拉伸强度差异。材料和方法。F344大鼠坐骨神经是新鲜的或去细胞的，并且测试整个（部分A）或横切并修复（部分B）。计算所有结构的平均极限应力、平均极限应变、杨氏模量和断裂总机械功。A-1组和A-2组的平均极限应变分别为0.480 +/- 0.117和0.810 +/- 0.114。A-1组和A-2组的杨氏模量分别为576 +/- 160和580 +/- 150 kPa。在A-1和A-2组中，标准化失效功分别为0.35 +/- 0.14和1.11 +/- 0.38 N。组B-1中的样本承受的平均极限应力为780 +/- 280 kPa。B-2组的试件承受的平均极限应力为405 ± 20 kPa。组B-1和B-2的平均极限应变分别为0.319 +/- 0.087和0.266 +/- 0.019。组B-1和B-2的杨氏模量分别为4，030 +/- 1360和2，290 +/- 280 kPa。组B-1和B-2的标准化失效功计算为0.22 +/- 0.04和0.11 +/- 0.02 N。结论。虽然足够强大的重建程序，脱细胞的周围神经相比，新鲜的神经无论是测试时，整个或横断和修复的抗张强度下降。(C)2003年爱思唯尔公司All rights reserved.
Background. Acellular nerve has been used in experimental models as a peripheral nerve substitute. Our objective was to determine the difference in tensile strength between fresh and chemically treated acellularized peripheral nerve.Materials and methods. F344 rat sciatic nerves were either fresh or acellularized and tested either whole (Part A) or transected and repaired (Part B). For all constructs, the mean ultimate stress, mean ultimate strain, Young's modulus, and total mechanical work to fracture were calculated.Results. The average ultimate strains for Groups A-1 and A-2 were 0.480 +/- 0.117 and 0.810 +/- 0.114, respectively. The Young's moduli in Groups A-1 and A-2 were 576 +/- 160 and 580 +/- 150 kPa, respectively. In Groups A-1 and A-2, the normalized work to failure was 0.35 +/- 0.14 and 1.11 +/- 0.38 N. The specimens in Group B-1 withstood an average ultimate stress of 780 +/- 280 kPa. The specimens in Group B-2 withstood an average ultimate stress of 405 20 kPa. The average ultimate strains for Groups B-1 and B-2 were 0.319 +/- 0.087 and 0.266 +/- 0.019, respectively. The Young's moduli in Groups B-1 and B-2 were 4,030 +/- 1360 and 2,290 +/- 280 kPa, respectively. The normalized work to failure in Groups B-1 and B-2 was calculated as 0.22 +/- 0.04 and 0.11 +/- 0.02 N.Conclusions. Although adequately robust for reconstructive procedures, the acellular peripheral nerve had decreased tensile strength compared with fresh nerve either when tested whole or when transected and repaired. (C) 2003 Elsevier Inc. All rights reserved.