OXYTOCIN AND OXYTOCIN RECEPTOR GENE-EXPRESSION IN THE REPRODUCTIVE-TRACT OF THE PREGNANT COW - RESCUE OF LUTEAL OXYTOCIN PRODUCTION AT TERM
OXYTOCIN AND OXYTOCIN RECEPTOR GENE-EXPRESSION IN THE REPRODUCTIVE-TRACT OF THE PREGNANT COW - RESCUE OF LUTEAL OXYTOCIN PRODUCTION AT TERM
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DOI：
10.1095/biolreprod53.3.553
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发表时间：
1995-09-01
影响因子：
3.6
通讯作者：
FUCHS, AR
中科院分区：
文献类型：
--
作者：
IVELL, R;RUST, W;FUCHS, AR
肽激素催产素在下丘脑和某些外周器官中产生。以前尚未检查过怀孕母牛下丘脑外激素合成的程度。在这项研究中，我们分析了怀孕牛生殖道和黄体的不同组织中是否存在编码催产素肽和催产素受体的mRNA，在子宫组织中，借助逆转录聚合酶链式反应方法只能偶尔检测到催产素mRNA，这意味着只有非常低的表达水平。肉阜的催产素基因表达水平始终较低，足月时表达上调。然而，在黄体中，足月时催产素基因表达水平显着，特别是在临产后。转录物水平足够高，可以通过 RNase 保护测定和 Northern 杂交进行测量；这些水平意味着在周期性妊娠和早期妊娠牛的黄体中观察到的催产素基因表达得到了挽救。在肽水平上，这种表达通过免疫组织化学得到证实。开发了一种灵敏的 RNase 保护测定法来检测编码催产素受体的转录本。在大多数子宫组织中均检测到转录本，其中在足月子宫内膜和子宫肌层中含量最高，在妊娠任何阶段的黄体中均未检测到转录本，在羊膜中也未检测到转录本。结果表明催产素在怀孕母牛的子宫内可能产生局部旁分泌作用。足月时黄体催产素的拯救可以起到补充垂体来源的循环激素的作用。
The peptide hormone oxytocin is produced both in the hypothalamus and in certain peripheral organs. The extent of extra-hypothalamic hormone synthesis in the pregnant cow has not previously been examined. In this study we have analyzed different tissues from the pregnant bovine reproductive tract and corpus luteum for the presence of mRNA encoding the oxytocin peptide as well as the oxytocin receptor, in uterine tissues oxytocin mRNA could only be detected sporadically with the help of a reverse transcription-polymerase chain reaction method, implying only very low levels of expression. The caruncles showed a consistently low level of oxytocin gene expression, up-regulated at term. However, in the corpus luteum there was a significant level of oxytocin gene expression at term, particularly following the onset of labor. The transcript levels were sufficiently high to be measurable by both RNase protection assay and by Northern hybridization;these levels imply a rescue of the oxytocin gene expression seen in the corpora lutea of cyclic and early pregnant cows. At the peptide level this expression was confirmed by immunohistochemistry. A sensitive RNase protection assay was developed to detect transcripts encoding ng the oxytocin receptor. Transcripts were detected in most uterine tissues, including the caruncles, with highest levels in the endometrium and myometrium at term, No transcripts could be detected in the corpus luteum at any stage of pregnancy, nor in the amnion. The results suggest the possibility of local, paracrine effects of oxytocin within the uterus of the pregnant cow. The rescue of luteal oxytocin at term could act to supplement the circulating hormone of pituitary origin.