Subcellular localization of the common shikimate-pathway enzymes in Pisum sativum L.
Subcellular localization of the common shikimate-pathway enzymes in Pisum sativum L.
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DOI：
10.1007/bf00393514
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发表时间：
1985-02
期刊：
影响因子：
4.3
通讯作者：
D. Mousdale;J. Coggins
中科院分区：
文献类型：
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作者：
D. Mousdale;J. Coggins
5-烯醇式莽草酸3-磷酸（EPSP）合酶（3-磷酸莽草酸1-羧基乙烯基转移酶; EC 2.5.1.19）、3-脱氢奎尼酸脱氢酶（EC 4.2.1.10）和莽草酸：NADP+氧化还原酶（EC 1.1.1.25）存在于通过蔗糖和改性二氧化硅密度梯度离心从豌豆幼苗提取物分离的完整叶绿体和根质体中。在年轻的时候（约。10天龄）幼苗芽的酶主要是叶绿体;高性能阴离子交换色谱解决未观察到的密度梯度纯化的叶绿体轻微同工酶活动。莽草酸途径的起始酶3-脱氧-d-阿拉伯-庚酮糖酸7-磷酸合酶（EC 4.1.2.15）也与完整的密度梯度纯化的叶绿体相关。3-脱氢奎尼酸合酶（EC 4.6.1.3）和莽草酸激酶（EC 2.7.1.71）与来自洗涤的叶绿体的基质制备物中的其它途径酶一起检测。质体EPSP合酶被微摩尔浓度的除草剂草甘膦抑制。
5-Enolpyruvylshikimate 3-phosphate (EPSP) synthase (3-phosphoshikimate 1-carboxyvinyltransferase; EC 2.5.1.19), 3-dehydroquinate dehydratase (EC 4.2.1.10) and shikimate: NADP+oxidoreductase (EC 1.1.1.25) were present in intact chloroplasts and root plastids isolated from pea seedling extracts by sucrose and modified-silica density gradient centrifugation. In young (approx. 10-d-old) seedling shoots the enzymes were predominantly chloroplastic; high-performance anion-exchange chromatography resolved minor isoenzymic activities not observed in density-gradientpurified chloroplasts. The initial enzyme of the shikimate pathway, 3-deoxy-d-arabino-heptulosonate 7-phosphate synthase (EC 4.1.2.15) was also associated with intact density-gradient-purified chloroplasts. 3-Dehydroquinate synthase (EC 4.6.1.3) and shikimate kinase (EC 2.7.1.71) were detected together with the other pathway enzymes in stromal preparations from washed chloroplasts. Plastidic EPSP synthase was inhibited by micromolar concentrations of the herbicide glyphosate.