LOCALIZATION OF IMMUNOREACTIVE SYNTHETIC ATRIAL NATRIURETIC FACTOR (ANF) IN THE HEART OF VARIOUS ANIMAL SPECIES
LOCALIZATION OF IMMUNOREACTIVE SYNTHETIC ATRIAL NATRIURETIC FACTOR (ANF) IN THE HEART OF VARIOUS ANIMAL SPECIES
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DOI：
10.1177/33.6.3158698
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发表时间：
1985-01-01
影响因子：
3.2
通讯作者：
CANTIN, M
中科院分区：
文献类型：
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作者：
CHAPEAU, C;GUTKOWSKA, J;CANTIN, M
用免疫组织化学和免疫细胞化学方法研究了心钠素C末端两个合成片段Arg 101-Tyr 126和Leu 94-Arg 109在哺乳动物(大鼠、小鼠、豚鼠、仓鼠、兔、猫、狗、人)和非哺乳类脊椎动物蟾蜍(Bufo Marinus)、蛙(Rana Catesbeiana)、鱼(Cyprinus carpio、Punus schwanenFeldi、Cichlasoma biocellatus、Carassius auratus)、蛇(Python Reticatus)和母鸡心脏中的定位。在兔体内制备抗ANF合成片段的抗体，并用于免疫荧光(Coons‘s技术)、免疫组织化学(未标记抗体技术)或免疫细胞化学(Protein A-Gold技术)。免疫荧光和未标记抗体技术得到的结果相似：抗Arg 101-Tyr 126 ANF的抗体可以在所有哺乳动物的心房中显示颗粒心肌细胞。虽然这种反应在大鼠和小鼠身上非常强烈，但在兔子身上就不那么强烈了，在包括人类在内的所有其他被研究物种中都非常弱。抗Leu 94-Arg 109 ANF的抗体仅在大鼠和小鼠中产生反应。在非哺乳动物脊椎动物中，这两种抗体在所有物种的心房中的反应总是比在脑室中的反应强烈得多。
The localization of 2 synthetic fragments of the C-terminal portion of atrial natriuretic factor: Arg 101-Tyr 126 which displays full biological activity and Leu 94-Arg 109 which is completely devoid of biological activity, was investigated by immunohisto- and immunocytochemical methods in the heart of mammals (rat, mouse, guinea pig, hamster, rabbit, cat, dog, man) and nonmammalian vertebrates toad (Bufo marinus), frog (Rana catesbeiana), fish (Cyprinus carpio, Puntius schwanenfeldi, Cichlasoma biocellatum, Carassius auratus), snake (Python reticulatus) and hen. Antibodies against the synthetic fragments of ANF were raised in rabbits and used either for immunofluorescence (Coons'' technique), immunohistochemistry (unlabeled antibody technique) or immunocytochemistry (protein A-gold technique). Results obtained by immunofluorescence and by the unlabeled antibody technique were similar: antibodies against Arg 101-Tyr 126 ANF allowed visualization of granulated cardiocytes in the atria of all mammals. While the reaction was very strong in rat and mouse, it was less so in the rabbit and very weak in all other species studied including man. Antibodies against Leu 94-Arg 109 ANF produced a reaction only in the rat and mouse. In nonmammalian vertebrates, the reaction was always much stronger in atria than ventricles of all species with both antibodies.