Lateral flow assay modified with time-delay wax barriers as a sensitivity and signal enhancement strategy
Lateral flow assay modified with time-delay wax barriers as a sensitivity and signal enhancement strategy
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DOI：
10.1016/j.bios.2020.112559
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发表时间：
2020-11-15
影响因子：
12.6
通讯作者：
Merkoci, Arben
中科院分区：
文献类型：
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作者：
Sena-Torralba, Amadeo;Duy Ba Ngo;Merkoci, Arben
侧流分析（LFA）的易用性，低成本和快速操作使其成为各种领域中最常见的护理点生物传感器。然而，它们通常的低灵敏度限制了它们在更具有挑战性的应用中的使用，在这些应用中需要检测低分析浓度。在这里，我们建议使用可溶性蜡屏障选择性地和暂时积累的测试线（TL）的顶部上的目标和标记纳米粒子。该延长的内部孵育步骤促进免疫复合物的形成，考虑到定量限，产生51.7倍的灵敏度增强，并且与用于人IgG（H-IgG）检测的常规LFA相比，信号增强高达96%。
The ease of use, low cost and quick operation of lateral flow assays (LFA) have made them some of the most common point of care biosensors in a variety of fields. However, their generally low sensitivity has limited their use for more challenging applications, where the detection of low analytic concentrations is required. Here we propose the use of soluble wax barriers to selectively and temporarily accumulate the target and label nanoparticles on top of the test line (TL). This extended internal incubation step promotes the formation of the immune-complex, generating a 51.7-fold sensitivity enhancement, considering the limit of quantification, and up to 96% signal enhancement compared to the conventional LFA for Human IgG (H-IgG) detection.