Selection of probiotic bacteria for prevention of allergic diseases: immunomodutation of neonatal dendritic cells
Selection of probiotic bacteria for prevention of allergic diseases: immunomodutation of neonatal dendritic cells
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DOI：
10.1111/j.1365-2249.2007.03421.x
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发表时间：
2007-08-01
影响因子：
4.6
通讯作者：
Rijkers, G. T.
中科院分区：
文献类型：
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作者：
Niers, L. E. M.;Hoekstra, M. O.;Rijkers, G. T.
通过施用益生菌来改变生命早期的肠道微生物群可能是预防过敏性疾病的潜在方法。为了选择用于体内目的的益生菌，我们研究了益生菌与新生儿树突状细胞 (DC) 相互作用的能力，并研究了随之而来的 T 细胞极化效应。未成熟DC由脐带血来源的单核细胞产生，并通过成熟因子(MF)、脂多糖(LPS)加MF以及两歧双歧杆菌、婴儿双歧杆菌、唾液乳杆菌、乳酸乳球菌单独或与MF组合诱导成熟。以 DC 和金黄色葡萄球菌肠毒素 B (SEB) 作为抗原刺激物共培养 12 天后，通过细胞内细胞因子染色测定自体 T 细胞产生的细胞因子。此外，用 CD3 和 CD28 单克隆抗体刺激细胞，并通过多重测定测量上清液中的细胞因子。益生菌菌株诱导 DC 部分成熟。当添加 MF 时，所有测试菌株均诱导 DC 完全成熟。与 MF 和 LPS 成熟 DC 刺激相比，用两歧双歧杆菌成熟 DC 刺激的 T 细胞培养物中产生白细胞介素 (IL)-4 的 T 细胞百分比较低，这与产生干扰素 (IFN)-γ 的 T 细胞百分比较高相一致。此外，与 MF 成熟 DC 相比，经两歧双歧杆菌成熟 DC 刺激的 T 细胞产生显着更多的 IL-10。双歧杆菌属的选定物种可在体外培养新生儿 DC 以使 T 细胞反应极化，因此可能成为用于过敏性疾病一级预防的候选者。
Modification of intestinal microbiota early in life by administration of probiotic bacteria may be a potential approach to prevent allergic disease. To select probiotic bacteria for in vivo purposes, we investigated the capacity of probiotic bacteria to interact with neonatal dendritic cells (DC) and studied the ensuing T cell polarizing effect. Immature DC were generated from cord blood-derived monocytes and maturation was induced by maturation factors (MF), lipopolysaccharide (LPS) plus MF and Bifidobacterium bifidum, B. infantis, Lactobacillus salivarius, Lactococcus lactis alone or combined with MF. After 12 days of co-culture with DC and Staphylococcus aureus enterotoxin B (SEB) as antigenic stimulus, cytokine production by autologous T cells was determined by intracellular cytokine staining. Additionally, cells were stimulated with CD3 and CD28 monoclonal antibodies and cytokines were measured in supernatants by multiplex assay. The probiotic strains induced partial maturation of DC. Full maturation of DC was induced for all strains tested when MF was added. The percentage of interleukin (IL)-4 producing T cells was lower in T cell cultures stimulated with B. bifidum matured DC compared to MF and LPS matured DC, which coincided with a higher percentage of interferon (IFN)-gamma-producing T cells. Furthermore, T cells stimulated by B. bifidum matured DC produced significantly more IL-10 compared to MF matured DC. Selected species of the Bifidobacterium genus prime in vitro cultured neonatal DC to polarize T cell responses and may therefore be candidates to use in primary prevention of allergic diseases.