Reduction of ferric iron by acidophilic heterotrophic bacteria:: evidence for constitutive and inducible enzyme systems in Acidiphilium spp.
Reduction of ferric iron by acidophilic heterotrophic bacteria:: evidence for constitutive and inducible enzyme systems in Acidiphilium spp.
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DOI：
10.1046/j.1365-2672.2002.01535.x
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发表时间：
2002-01-01
影响因子：
4
通讯作者：
Bridge, TAM
中科院分区：
文献类型：
--
作者：
Johnson, DB;Bridge, TAM
目的：为了比较两种专性嗜酸异养细菌，嗜酸嗜酸菌和嗜酸菌SJH，还原三价铁的能力，当生长在不同的培养条件下ferrous.Methods和结果：细菌生长在分批培养，在不同的通气状态下，在亚铁或三价铁的存在下。发酵培养的嗜酸菌SJH还原三价铁的比速率不受溶解氧浓度的影响，而嗜酸菌SJH还原铁的比速率受溶解氧浓度的影响。嗜酸菌的生长高度依赖于生长培养基中的DO浓度。存在的铁的离子形式（亚铁或三价铁）对收获的细胞还原三价铁的能力具有最小的影响。无论生长培养基的DO状态如何，嗜酸菌SJH的全细胞蛋白质谱都非常相似，而在嗜酸菌SJH中存在额外的蛋白质。结论：嗜酸菌SJH的异化还原是组成性的，而嗜酸杆菌的异化还原是诱导性的。研究的意义和影响：嗜酸菌还原三价铁的作用。可发生在含氧以及缺氧的酸性环境中。这将降低生物修复系统的有效性，其中从污染的沃茨中去除铁是通过金属的氧化和沉淀来介导的。
Aims: To compare the abilities of two obligately acidophilic heterotrophic bacteria, Acidiphilium acidophilum and Acidiphilium SJH, to reduce ferric iron to ferrous when grown under different culture conditions.Methods and Results: Bacteria were grown in batch culture, under different aeration status, and in the presence of either ferrous or ferric iron. The specific rates of ferric iron reduction by fermenter-grown Acidiphilium SJH were unaffected by dissolved oxygen (DO) concentrations, while iron reduction by A. acidophilum was highly dependent on DO concentrations in the growth media. The ionic form of iron present (ferrous or ferric) had a minimal effect on the abilities of harvested cells to reduce ferric iron. Whole cell protein profiles of Acidiphilium SJH were very similar, regardless of the DO status of the growth medium, while additional proteins were present in A. acidophilum grown microaerobically compared with aerobically-grown cells.Conclusions: The dissimilatory reduction of ferric iron is constitutive in Acidiphilium SJH while it is inducible in A. acidophilum.Significance and Impact of the Study: Ferric iron reduction by Acidiphilium spp. may occur in oxygen-containing as well as anoxic acidic environments. This will detract from the effectiveness of bioremediation systems where removal of iron from polluted waters is mediated via oxidation and precipitation of the metal.