DEVELOPMENT AND EVALUATION OF AN EXPERIMENTAL VACCINATION PROGRAM USING A LIVE AVIRULENT SALMONELLA-TYPHIMURIUM STRAIN TO PROTECT IMMUNIZED CHICKENS AGAINST CHALLENGE WITH HOMOLOGOUS AND HETEROLOGOUS SALMONELLA SEROTYPES
DEVELOPMENT AND EVALUATION OF AN EXPERIMENTAL VACCINATION PROGRAM USING A LIVE AVIRULENT SALMONELLA-TYPHIMURIUM STRAIN TO PROTECT IMMUNIZED CHICKENS AGAINST CHALLENGE WITH HOMOLOGOUS AND HETEROLOGOUS SALMONELLA SEROTYPES
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DOI：
10.1128/iai.62.12.5519-5527.1994
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发表时间：
1994-12-01
影响因子：
3.1
通讯作者：
CURTISS, R
中科院分区：
文献类型：
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作者：
HASSAN, JO;CURTISS, R
在几种疫苗接种策略中使用了稳定的无毒力、遗传修饰的Delta cya Delta crp鼠伤寒沙门氏菌疫苗株chi 3985，以评价其在控制鸡沙门氏菌感染中的用途。用10（8）CFU chi 3985口服接种1日龄和14日龄的鸡，可防止脾、卵巢和腔上囊的侵入，以及用10（6）CFU来自B组的强毒同源沙门氏菌菌株攻击的鸡的回肠和盲肠的定植。用来自C、D和E组的异源沙门氏菌菌株攻击的鸡保护脾脏和卵巢免受内脏侵入，而在接种疫苗的鸡中减少了腔上囊的侵入和回肠和盲肠的定植。在2周龄和4周龄时口服疫苗接种诱导了针对毒性B组沙门氏菌血清型攻击的极好保护，以及针对D组或E组沙门氏菌血清型攻击的非常好的保护，而针对C组沙门氏菌血清型攻击的保护是边缘的，但是显著的，在2周龄和4周龄接种疫苗也保护接种的鸡抵抗10（8）CFU的高侵袭性S.鼠伤寒沙门氏菌或S.有很多菌株。用chi 3985免疫的鸡抵抗同源和异源沙门氏菌血清型攻击的保护作用是突出的，并且在用10（8）CPU的高侵袭性沙门氏菌攻击的鸡中抵抗卵巢侵袭的完全保护作用是显著的。鼠伤寒沙门氏菌或S.沙门氏菌菌株表明，用chi 3985接种鸡可以补充目前基于卫生和环境卫生的沙门氏菌控制措施。本文报道了一个突破，在使用活的无毒疫苗，以控制沙门氏菌携带者在增厚。
A stable live avirulent, genetically modified Delta cya Delta crp Salmonella typhimurium vaccine strain, chi 3985, was used in several vaccination strategies to evaluate its use in the control of Salmonella infection in chickens. Oral vaccination of chickens at 1 and at 14 days of age with 10(8) CFU of chi 3985 protected against invasion of spleen, ovary, and bursa of Fabricius and colonization of the ileum and cecum in chickens challenged with 10(6) CFU of virulent homologous Salmonella strains from group B. Chickens challenged with heterologous Salmonella strains from groups C, D, and E were protected against visceral invasion of spleen and ovary, while invasion of the bursa of Fabricius and colonization of ileum and cecum was reduced in vaccinated thickens. Oral vaccination at 2 and at 4 weeks of age induced an excellent protection against challenge with virulent group B Salmonella serotypes and very good protection against challenge with group D or E Salmonella serotypes, while protection against challenge with group C Salmonella serotypes was marginal but significant, Vaccination at 2 and at 4 weeks of age also protected vaccinated chickens against challenge with 10(8) CFU of highly invasive S. typhimurium or S. enteritidis strains. The protection of chickens vaccinated with chi 3985 against challenge with homologous and heterologous Salmonella serotypes is outstanding and the complete protection against ovarian invasion in chickens challenged with 10(8) CPU of highly invasive S. typhimurium or S. enteritidis strains suggests that vaccination of chickens with chi 3985 can complement the present hygiene- and sanitation-based Salmonella control measures. This paper reports a breakthrough in the use of live avirulent vaccine to control Salmonella carriers in thickens.