Quantitative Assessment of Nanoparticle Biodistribution by Fluorescence Imaging, Revisited.
Quantitative Assessment of Nanoparticle Biodistribution by Fluorescence Imaging, Revisited.
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DOI：
10.1021/acsnano.8b02881
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发表时间：
2018-07-24
期刊：
影响因子：
17.1
通讯作者：
Yeo Y
中科院分区：
文献类型：
--
作者：
Meng F;Wang J;Ping Q;Yeo Y
基于荧光的全身成像广泛用于小动物中纳米颗粒（NP）的评价，通常与定量分析相结合以指示其在全身给药后的时空分布。基本假设是荧光标记代表NP，并且强度随着NP和/或在感兴趣区域中累积的标记染料的量而增加。我们制备了DiR负载的聚乳酸-羟基乙酸共聚物（PLGA）纳米颗粒，具有不同的表面层（具有和不具有叶酸末端的聚乙二醇），并通过近红外荧光全身成像比较了叶酸受体表达肿瘤小鼠模型中荧光信号的分布。出乎意料的是，我们观察到，与表面配体相比，DiR负载的荧光分布模式的差异更显著，用相同类型的NP得出相反的结论（肿瘤特异性递送与主要的肝脏积累）。DiR负载的PLGA纳米颗粒的分析表明，荧光淬灭，去淬灭和信号饱和，这与染料含量和局部NP浓度的增加，是负责冲突的解释。这项研究强调了在全身成像的定量分析中验证NP的荧光标记的迫切需要。根据我们的观察，我们提出了建议，为未来的全身荧光成像在体内评价NP的行为。
Fluorescence-based whole body imaging is widely used in the evaluation of nanoparticles (NPs) in small animals, often combined with quantitative analysis to indicate their spatiotemporal distribution following systemic administration. An underlying assumption is that the fluorescence label represents NPs and the intensity increases with the amount of NPs and/or the labeling dyes accumulated in the region of interest. We prepare DiR-loaded poly(lactic-co-glycolic acid) (PLGA) NPs with different surface layers (polyethylene glycol with and without folate terminus) and compare the distribution of fluorescence signals in a mouse model of folate-receptor expressing tumor by near infrared fluorescence whole body imaging. Unexpectedly, we observe that fluorescence distribution patterns differ far more dramatically with DiR loading than with the surface ligand, reaching opposite conclusions with the same type of NPs (tumor-specific delivery vs. predominant liver accumulation). Analysis of DiR-loaded PLGA NPs reveal that fluorescence quenching, dequenching and signal saturation, which occur with the increasing dye content and local NP concentration, are responsible for the conflicting interpretations. This study highlights the critical need for validating fluorescence labeling of NPs in the quantitative analysis of whole body imaging. In light of our observation, we make suggestions for future whole body fluorescence imaging in the in vivo evaluation of NP behaviors.
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影响因子：
14
作者：
Guo, Jintang;Hong, Hao;Chen, Guojun;Shi, Sixiang;Zheng, Qifeng;Zhang, Yin;Theuer, Charles P.;Barnhart, Todd E.;Cai, Weibo;Gong, Shaoqin
通讯作者：
Gong, Shaoqin
影响因子：
16.6
作者：
Guo P;Liu D;Subramanyam K;Wang B;Yang J;Huang J;Auguste DT;Moses MA
通讯作者：
Moses MA
影响因子：
62.1
作者：
Kobayashi, Hisataka;Ogawa, Mikako;Alford, Raphael;Choyke, Peter L.;Urano, Yasuteru
通讯作者：
Urano, Yasuteru
影响因子：
82.9
作者：
Bremer, C;Tung, CH;Weissleder, R
通讯作者：
Weissleder, R
影响因子：
56.9
作者：
Ejima, Hirotaka;Richardson, Joseph J.;Caruso, Frank
通讯作者：
Caruso, Frank