Ethanol and acetaldehyde: in vivo quantitation and effects on cholinergic function in rat brain.
Ethanol and acetaldehyde: in vivo quantitation and effects on cholinergic function in rat brain.
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DOI：
10.1002/9780470511848.ch10
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发表时间：
2007-04
影响因子：
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通讯作者：
M. Jamal;K. Ameno;U. Ikuo;M. Kumihashi;Weihuan Wang;I. Ijiri
中科院分区：
文献类型：
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作者：
M. Jamal;K. Ameno;U. Ikuo;M. Kumihashi;Weihuan Wang;I. Ijiri
首先，乙醇（EtOH）和乙醛的水平，同时确定在自由活动的大鼠纹状体后，他们的主要氧化酶抑制剂，然后由EtOH。结果表明，乙醛存在于氨腈（CY）+ EtOH、CY + 4-甲基吡唑（4-MP）+ EtOH和CY +叠氮化钠+ EtOH基团中。CY + EtOH诱导的乙醛峰值水平为195.2 +/- 19.4 μ M，该值显著高于其他组。在CY + 4-MP + EtOH组中，EtOH峰值水平为25.9 +/- 2.3mM，并且该水平显著高于EtOH中的值。在研究的任何其他组中，均未发现脑EtOH水平的显著差异。第二，采用RT-PCR和Western blot方法研究乙醇和乙醛对高乙酰丙酮酸产生大鼠额叶皮层和海马胆碱乙酰转移酶（ChAT）和乙酰胆碱酯酶（AChE）的影响。结果表明，乙醇和乙醛降低脑中乙醇给药后40和240分钟的ChAT表达。乙酰丙酮诱导的ChAT表达的减少显著高于EtOH诱导的。AChE表达无明显变化。该研究表明，过氧化氢酶作出了显着的贡献，乙醛在大鼠脑中的形成，和乙醇和乙醛减少ChAT表达在40和240分钟后，乙醇给药。
First, ethanol (EtOH) and acetaldehyde levels were determined simultaneously in the striatum of free-moving rats after administration of their major oxidative enzyme inhibitors followed by EtOH. The results showed that acetaldehyde was present in the cyanamide (CY) + EtOH, CY + 4-methylpyrazole (4-MP) + EtOH and CY + sodium azide + EtOH groups. The CY + EtOH-induced peak acetaldehyde level was 195.2 +/- 19.4 microM, and this value was significantly higher than those in the other groups. The peak EtOH level was 25.9 +/- 2.3mM in the CY + 4-MP + EtOH group, and this level was considerably higher than the value in EtOH. No significant difference in brain EtOH levels was found in any of the other groups studied. Second, the effects of EtOH and acetaldehyde on choline acetyltransferase (ChAT) and acetylcholinesterase (AChE) were investigated in the frontal cortex and hippocampus of high acetaldehyde-producing rats using RT-PCR and Western blot. The results showed that EtOH and acetaldehyde decreased ChAT expression at 40 and 240 min after EtOH dosing in the brain. The acetaldehyde-induced reduction in ChAT expression was significantly higher than that induced by EtOH. No remarkable alteration of AChE expression was observed. The study suggested that catalase made a significant contribution to acetaldehyde formation in the rat brain, and that EtOH and acetaldehyde decreased ChAT expression at 40 and 240 min after EtOH dosing.