Modulation of dopamine uptake in rat nucleus accumbens: effect of specific dopamine receptor antagonists and sigma ligands.
Modulation of dopamine uptake in rat nucleus accumbens: effect of specific dopamine receptor antagonists and sigma ligands.
复制标题
大鼠伏隔核多巴胺摄取的调节：特异性多巴胺受体拮抗剂和西格玛配体的作用。
DOI：
10.1016/s0304-3940(01)02209-1
复制
发表时间：
2001
影响因子：
2.5
通讯作者：
Miller,C
中科院分区：
文献类型：
--
作者：
Thompson,TL;Bridges,S;Miller,C
多巴胺（DA）拮抗剂和sigma受体配体改变[3 H]-DA摄取的能力进行了检查，使用突触体从雌性大鼠的脑桥核制备。与对σ（林卡唑、氟哌啶醇和螺哌隆）受体具有高亲和力的化合物预孵育产生3 H-DA摄取的剂量依赖性抑制。纯DA D2受体拮抗剂舒必利则无此作用。与此相反，DA摄取增强响应于（+）-3-（3-羟基苯基）-N-（1-丙基）哌啶，一种混合的σ受体拮抗剂和DA D2受体激动剂。类似地，SKF-10，047（一种选择性σ受体激动剂）和孕酮（一种σ受体的假定内源性配体）显著增加3 [H]-DA摄取。这些数据表明，σ和DA配体在调节多巴胺的吸收在丘脑核中的潜在作用。
The ability of dopamine (DA) antagonists and sigma receptor ligands to alter [3H]-DA uptake was examined using synaptosomes prepared from the nucleus accumbens of female rats. Pre-incubation with compounds having a high affinity for sigma (rimcazole, haloperidol, and spiperone) receptors produced dose dependent inhibition of3H-DA uptake. Sulpiride, a pure DA D2antagonist had no effect. In contrast, DA uptake was potentiated in response to (+)-3-(3-hydroxyphenyl)-N-(1-propyl)piperidine, a mixed sigma receptor antagonist and DA D2receptor agonist. Similarly, SKF-10,047, a selective sigma receptor agonist, and progesterone, a putative endogenous ligand for the sigma receptor, produced significant increases in3[H]-DA uptake. These data suggest a potential role for sigma and DA ligands in the regulation of DA uptake in the nucleus accumbens.