Gel Electrophoresis Analysis of rDNA Instability in Saccharomyces cerevisiae
Gel Electrophoresis Analysis of rDNA Instability in Saccharomyces cerevisiae
复制标题
酿酒酵母 rDNA 不稳定性的凝胶电泳分析
DOI：
10.1007/978-1-0716-0644-5_28
复制
发表时间：
2021
影响因子：
--
通讯作者：
Takehiko Kobayashi
中科院分区：
文献类型：
--
作者：
Mariko Sasaki;Takehiko Kobayashi
核糖体RNA （rDNA）序列是出芽酵母基因组中最丰富的重复元件，形成约100-200个拷贝的串联簇。细胞经常改变它们的rDNA拷贝数，使rDNA成为芽殖酵母基因组中最不稳定的区域。rDNA区域经历了程序化复制叉阻滞和随后形成的DNA双链断裂（DSBs），这是rDNA不稳定的主要驱动因素。rDNA区域提供了一个独特的系统来理解响应复制叉阻止的机制以及调节重复不稳定性的机制。本章描述了评估rDNA不稳定性的三种方法。
The ribosomal RNA (rDNA) sequence is the most abundant repetitive element in the budding yeast genome and forms a tandem cluster of ~100–200 copies. Cells frequently change their rDNA copy number, making rDNA the most unstable region in the budding yeast genome. The rDNA region experiences programmed replication fork arrest and subsequent formation of DNA double-strand breaks (DSBs), which are the main drivers of rDNA instability. The rDNA region offers a unique system to understand the mechanisms that respond to replication fork arrest as well as the mechanisms that regulate repeat instability. This chapter describes three methods to assess rDNA instability.