Hermissenda crassicornis larvae metamorphose in laboratory in response to artificial and natural inducers.
Hermissenda crassicornis larvae metamorphose in laboratory in response to artificial and natural inducers.
复制标题
Hermissenda crassicornis 幼虫在实验室中对人工和天然诱导剂做出反应而发生变态。
DOI：
10.1086/bblv187n2p252
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发表时间：
1994
期刊：
影响因子：
--
通讯作者：
Kuzirian,AM
中科院分区：
文献类型：
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作者：
Avila,C;Arigue,A;Tamse,CT;Kuzirian,AM
粗角软体动物Hermissenda crassicornis(Eschcitz，1831)是一种在神经生物学研究中被用作模型的异桥软体动物，正在我们的实验室进行培养。反鱼养殖业的一个主要目标是提高变态丝状幼体到稚贝的比率和存活率。几位作者报道了诱导后桥幼虫变态的天然和人工物质的影响(参考文献1，2，3)。在3)中。因此，要提高艾米森达的变形率。我们已经测试了下列可能的诱导剂的效果：L-谷氨酸、氯化镁、生物膜和水生大黄鱼的全部或部分水提物，目前在我们实验室使用的天然诱导剂[4]。艾美森达幼虫取自本实验室培养物。通过标准培养方法获得合格幼虫(4)。管状线虫由海洋生物实验室海洋资源部提供。对于表I所列的每个处理，分别用过滤海水(FSW，目数：0.2 pm)、乙二胺四乙酸二钠(0.25 mg/L)和微藻(作为食物)(4)制备两个重复的50毫升试管，每个试管包含30只幼虫(45日龄)。将试管装在瓶子里，在12℃的滚瓶系统中旋转，阴性对照组不接受额外处理；阳性对照组加入3-4个息肉和管状茎。将全部或选定的部分管状线虫(相当于阳性对照的量)均质在3mlFSW中，制备水提物，加入全强度或稀释50%。生物膜是在流动海水中放置一个月的载玻片上自然生长的。将幼虫暴露于其中一种处理10天。随后，它们都被改为FSW(加入EDTA和藻类)，再加上管藻，用于(1)喂养变态幼虫，(2)测试对初始处理方案无效的幼虫的变态能力。每种潜在诱导剂、其浓度和变态成功率列于表I。变态开始之前的最短潜伏期为4天。在阴性对照组中确实发生了一些自发的变态，其速度高于预期。孤立的小管状息肉不会引起
Hermissenda crassicornis (Eschscholtz, 1831), an opisthobranch mollusc used as a model in neurobiological studies, is being cultured in our laboratory. A major aim in opisthobranch mariculture is to increase the rate and survival of metamorphic veliger larvae to juvenile slugs. Several authors have reported on the effects of natural and artificial substances that have induced opisthobranch larvae to metamorphose (1, 2, 3, and refs. in 3). Therefore, to improve the rate of metamorphosis in Hermissenda. we have tested the efficacy of the following putative inducers: L-glutamic acid, magnesium chloride, biofilms, and aqueous extracts of whole, or parts of, the hydroid Tubularia crocea, the natural inducer currently used in our laboratory (4). Hermissenda larvae were obtained from our laboratory cultures. Competent larvae were obtained by standard culture methods (4). Tubularia was provided by the Marine Resources Department of the Marine Biological Laboratory. For each treatment listed in Table I, two replicate 50-ml tubes containing 30 larvae each (45 days old) were prepared with filtered seawater (FSW, mesh: 0.2 pm), EDTA (0.25 mg/l), and microalgae (as food)(4). The tubes were held in bottles and rotated in a rollerbottle system at 12 C. Negative controls received no additional treatment; positive controls had 3-4 polyps and stalks of Tubularia added. Whole or selected portions of Tubularia (amounts equal to the positive control) were homogenized in 3 ml of FSW to prepare aqueous extracts, that were added either full strength or diluted 50%. Biofilms were naturally grown on slides kept in running seawater for one month. Larvae were exposed to one of the treatments for 10 days. Subsequently, they were all changed to FSW (with EDTA and algae), plus Tubularia to (1) feed the metamorphosed juveniles, and (2) to test the metamorphic competency of those larvae that failed to respond to the initial treatment regime.Each potential inducer, its concentration, and its metamorphic success rate are listed in Table I. The minimum latency before the onset of metamorphosis was 4 days. Some spontaneous metamorphosis did occur in the negative controls, at a higher than expected rate. Isolated Tubularia polyps did not induce