Transcriptional profiling and growth kinetics of endothelium reveals differences between cells derived from porcine aorta versus aortic valve
Transcriptional profiling and growth kinetics of endothelium reveals differences between cells derived from porcine aorta versus aortic valve
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DOI：
10.1016/s1010-7940(03)00408-1
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发表时间：
2003-10-01
影响因子：
3.4
通讯作者：
Byrne, JG
中科院分区：
文献类型：
--
作者：
Farivar, RS;Cohn, LH;Byrne, JG
目的：瓣膜组织和主动脉作为新鲜同种移植物或冷冻保存的同种异体移植物时以不同的速率钙化。此外。瓣膜内皮细胞和主动脉内皮细胞之间的差异没有得到很好的认识。我们建立了来自猪的瓣膜和主动脉内皮细胞的原代培养物，并测试了在各种细胞外基质上的转录和增殖差异。方法：对原代培养的猪主动脉和瓣膜内皮细胞进行转录谱分析。我们从两种细胞类型中提取总RNA并创建cDNA文库。我们对847个在信号转导通路中重要的基因进行了评分，并测量了它们在瓣膜和主动脉内皮细胞上的表达。为了确定主动脉和瓣膜细胞之间是否存在功能差异，通过对各种细胞外基质进行细胞计数来确定它们的生长速率。结果：在847个基因中，69个（8.1%）在主动脉内皮细胞上有转录活性，89个（10.5%）在瓣膜内皮细胞上有转录活性。两种细胞类型共有55个基因，占主动脉内皮细胞激活基因的79.7%（55/69），占瓣膜内皮细胞激活基因的61.8%（55/89）。显着的特点，分析包括肝配体和受体的特异性细胞类型，一个潜在的成纤维细胞生长因子自分泌环在两种细胞类型。以及瓣膜细胞中血小板衍生生长因子受体的上调。主动脉内皮细胞中血管内皮细胞生长因子-B和血管细胞粘附分子表达上调。增殖分析显示，瓣膜内皮细胞比主动脉内皮细胞生长更快（对照组的12倍）（对照组的3倍）。此外，瓣膜内皮细胞在明胶或胶原上增殖最快，而主动脉内皮细胞在赖氨酸或层粘连蛋白上增殖最快。结论：瓣膜和主动脉内皮细胞具有不同的转录和增殖特征。这些差异的知识可能是一个可利用的策略，在未来的合理设计的人工工程瓣膜表面和瓣膜抗原性的研究。免疫原性和结构失效。(C)2003 Elsevier B. V.保留所有权利。
Objective: Valvular tissue and aorta calcify at different rates when placed as fresh homografts or cryopreserved allografts. Furthermore. differences between valvular endothelial cells and aortic endothelial cells are not well appreciated. We established primary cultures of valve and aortic endothelial cells derived from swine and tested transcriptional and proliferative differences on various extracellular matrices. Methods: Transcriptional profiling was performed on primary Cultures of porcine valve and aortic endothelial cells. We extracted total RNA from both cell types and created cDNA libraries. We scored for 847 genes important in signal transduction pathways, and measured their expression on valve and aortic endothelial cells. To determine if there were functional differences between aortic and valvular cells, their growth rate was determined by cell counting on various extracellular matrices. Results: Of 847 genes investigated, 69 (8.1 %) were transcriptionally active on aortic endothelial cells and 89 (10.5%) on valve endothelial cells. Common to both cell types were 55 genes, which represents 79.7% (55/69) of activated genes on aortic endothelial cells and 61.8% (55/89) of those in valve endothelial cells. Remarkable features of the analysis included Ephrin ligand and receptor specificity for cell type, a potential fibroblast growth factor autocrine loop in both cell types. as well as upregulation of the platelet-derived growth factor receptor in valvular cells. Aortic endothelial cells were noteworthy of upregulation of vascular endothelial cell growth factor-B and vascular cell adhesion molecule. Proliferation analysis revealed that valve endothelial cells grew more rapidly (12-fold over control) than aortic endothelial cells (3-fold over control). Furthermore, valve endothelial cells proliferated most rapidly on gelatin or collagen, whereas aortic endothelial cells were most proliferative on lysine or laminin. Conclusions: Valve and aortic endothelial cells have different transcriptional and proliferative profiles. The knowledge of these differences may be an exploitable strategy in the future rational design of artificially engineered valve Surfaces and in the study of the valve antigenicity. immunogenicity and structural failure. (C) 2003 Elsevier B.V. All rights reserved.