Dictyobacter aurantiacus gen. nov., sp. nov., a member of the family Ktedonobacteraceae, isolated from soil, and emended description of the genus Thermosporothrix.
Dictyobacter aurantiacus gen. nov., sp. nov., a member of the family Ktedonobacteraceae, isolated from soil, and emended description of the genus Thermosporothrix.
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DOI：
10.1099/ijsem.0.001985
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发表时间：
2017-07
影响因子：
2.8
通讯作者：
S. Yabe;Y. Sakai;Keietsu Abe;A. Yokota;A. Take;A. Matsumoto;A. Sugiharto;D. Susilowati;Moriyuki Hamada;K. Nara;I. Made Sudiana;S. Otsuka
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文献类型：
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作者：
S. Yabe;Y. Sakai;Keietsu Abe;A. Yokota;A. Take;A. Matsumoto;A. Sugiharto;D. Susilowati;Moriyuki Hamada;K. Nara;I. Made Sudiana;S. Otsuka
从印度尼西亚西爪哇Gunung Salak(Mountain Salak)的水稻土壤中分离到一株中温、革兰氏染色阳性的芽胞形成细菌，该细菌形成分枝菌丝。该菌株定名为S-27T，生长温度为20~37℃，最适生长温度为25~30℃，15℃或45℃时不生长，最适生长pH范围为3.5~8.6，最适pH为6.0，pH 3.0或9.2时不生长。菌株S-27T能够降解淀粉、纤维素和木聚糖等多糖。菌株S-27T的G+C含量为55.7mol%。主要脂肪酸为异C17：0和C16：12-OH，主要为MK-9(H2)。菌株S-27T的细胞壁含有d-谷氨酸、甘氨酸、L-丙氨酸、d-丙氨酸、L-鸟氨酸和β-丙氨酸，摩尔比为1：1.6：1.4：0.6：0.9：1.1。极性脂由磷脂酰甘油、磷脂酰肌醇和两种糖脂组成。细胞壁糖主要为阿拉伯糖。基于16SRRNA基因序列的详细系统发育分析表明，S-27T菌株属于克氏杆菌目，与克氏杆菌消旋体SOSP1-21T亲缘关系最近(序列同源性为89.6%)。根据其化学分类、表型特征和系统发育地位，我们认为S-27T是一个新的属和种，我们将其命名为Dictybacter aurantiacus gen。Nov.，sp.11月模式菌株为S-27T(=NBRC109595T=INACCB312T)。并对热孢子丝属的描述进行了校订。
A mesophilic, Gram-stain-positive, spore-forming bacterium that formed branched mycelia was isolated from paddy soil in Gunung Salak (Mount Salak), West Java, Indonesia. This strain, designated S-27T, grew at temperatures between 20 and 37 °C; the optimum growth temperature was 25 to 30 °C, and no growth was observed at 15 or 45 °C. The pH range for growth was pH 3.5 to 8.6; the optimum pH was 6.0, and no growth was observed at pH 3.0 or 9.2. Strain S-27T was able to hydrolyse polysaccharides such as starch, cellulose and xylan. The G+C content of the DNA of strain S-27T was 55.7 mol%. The major fatty acids were iso-C17 : 0 and C16 : 1 2-OH, and the major menaquinone was MK-9 (H2). The cell wall of strain S-27T contained d-glutamic acid, glycine, l-alanine, d-alanine, l-ornithine and β-alanine in a molar ratio of 1.0 : 1.6 : 1.4 : 0.6 : 0.9 : 1.1. The polar lipids consisted of phosphatidylglycerol, phosphatidylinositol and two glycolipids. The major cell-wall sugar was arabinose. Detailed phylogenetic analysis based on 16S rRNA gene sequences indicated that strain S-27T belongs to the order Ktedonobacterales and is most closely related to Ktedonobacter racemifer SOSP1-21T (89.6 % sequence identity). On the basis of its chemotaxonomic and phenotypic features and phylogenetic position, we concluded that strain S-27T represents a novel genus and species, for which we propose the name Dictyobacter aurantiacus gen. nov., sp. nov. The type strain of Dictyobacter aurantiacus is strain S-27T (=NBRC 109595T=InaCC B312T). Emendation of the description of the genus Thermosporothrix is also provided.