Plant volatile organic compound (E)-2-hexenal facilitates Botrytis cinerea infection of fruits by inducing sulfate assimilation
Plant volatile organic compound (E)-2-hexenal facilitates Botrytis cinerea infection of fruits by inducing sulfate assimilation
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植物挥发性有机化合物(E)-2-己烯醛通过诱导硫酸盐同化促进果实灰霉病感染
DOI：
10.1111/nph.17378
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发表时间：
2021
期刊：
影响因子：
9.4
通讯作者：
Fang Weiguo
中科院分区：
文献类型：
--
作者：
Xu Yanqun;Tong Zhichao;Zhang Xiaochen;Zhang Xing;Luo Zisheng;Shao Wenyong;Li Li;Ma Quan;Zheng Xiaodong;Fang Weiguo
植物-真菌相互作用的研究是植物科学中最有趣的领域之一。然而，植物挥发性有机化合物在军备竞赛中的作用在很大程度上还不清楚。在对植物挥发性物质进行精确定量的基础上，我们发现，当植物挥发性有机化合物(E)-2-己烯醛的浓度与草莓和番茄果实组织中的浓度相似或低于草莓和番茄果实组织中的浓度时，植物挥发性有机化合物(E)-2-己烯醛上调了植物病原菌Botrytis cinerea孢子和菌丝中的硫酸盐同化作用。这种上调与植物中硫源的类型无关，可以在无机硫酸盐和有机硫源的存在下实现。利用真菌缺失突变体，我们进一步发现，硫酸盐同化参与了番茄和草莓果实的侵染，病情严重程度与果实中硫酸盐含量成正比。在感染之前和感染期间，(E)-2-己烯醛诱导利用植物硫酸盐BYB。灰霉菌通过增强其对氧化胁迫的耐受性来促进其发病。这项工作为植物挥发物在植物与真菌病原菌相互作用中的作用提供了新的见解，并强调了寄主中硫水平在预防灰霉病中的重要性。
Investigation into plant–fungal pathogen interactions is one of the most interesting fields in plant sciences. However, the roles of plant volatile organic compounds in the arms race are still largely unknown.Based on precise quantification of plant volatiles, we discovered that the plant volatile organic compound (E)‐2‐hexenal, at concentrations that were similar to or lower than those in tissues of strawberry and tomato fruits, upregulates sulfate assimilation in spores and hyphae of the phytopathogenic fungusBotrytis cinerea. This upregulation is independent of the types of sulfur sources in the plant and can be achieved in the presence of inorganic sulfate and organic sulfur sources.Using the fungal deletion mutants, we further found that sulfate assimilation is involved in the infection of tomato and strawberry fruits byB. cinerea, and that the severity of the disease is proportional to the sulfate content in the fruits. Both before and during the infection, (E)‐2‐hexenal induced utilisation of plant sulfate byB. cinereafacilitates its pathogenesis through enhancing its tolerance to oxidative stress.This work provides novel insights into the role of plant volatiles in plant–fungal pathogen interaction and highlights the importance of sulfur levels in the host in the prevention of grey mould disease.